Detailed information of TA system TA025410

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 23208..23700
• Replicon: plasmid pZYMOP02
• Accession: NC_015716
• Organism: Zymomonas mobilis subsp. pomaceae ATCC 29192

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: F8EWJ3
• Locus tag: ZYMOP_RS09100
• Protein ID: WP_013945643.1
• Coordinates: 23416..23700 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: F8EWJ2
• Locus tag: ZYMOP_RS09095
• Protein ID: WP_013945642.1
• Coordinates: 23208..23432 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ZYMOP_RS09375	18657..18899	+	243	Protein_12	transposase	-
ZYMOP_RS09270	18871..19395	+	525	WP_049778896.1	hypothetical protein	-
ZYMOP_RS09075	19380..19835	+	456	WP_049778897.1	hypothetical protein	-
ZYMOP_RS09090	21062..22057	+	996	WP_013945641.1	NADP-dependent oxidoreductase	-
ZYMOP_RS09380	22375..22638	-	264	WP_080558593.1	Na+/H+ antiporter NhaA	-
ZYMOP_RS09095	23208..23432	+	225	WP_013945642.1	TraY domain-containing protein	Antitoxin
ZYMOP_RS09100	23416..23700	+	285	WP_013945643.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ZYMOP_RS09105	23835..24467	+	633	WP_013945644.1	ParA family protein	-
ZYMOP_RS09110	24464..24739	+	276	WP_013945645.1	hypothetical protein	-
ZYMOP_RS09115	25422..25667	+	246	WP_049778898.1	helix-turn-helix domain-containing protein	-
ZYMOP_RS09125	27019..27339	-	321	WP_013945648.1	DUF1870 family protein	-
ZYMOP_RS09130	27348..27728	-	381	WP_013945649.1	type II toxin-antitoxin system death-on-curing family toxin	-
ZYMOP_RS09135	27725..27955	-	231	WP_013945650.1	hypothetical protein	-
ZYMOP_RS09140	28098..28280	-	183	WP_013945651.1	hypothetical protein	-
ZYMOP_RS09475	28465..28629	+	165	WP_013945652.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..34161	34161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10877.88 Da
• Isoelectric Point: 10.9851

>T25410 WP_013945643.1 NC_015716:23416-23700 [Zymomonas mobilis subsp. pomaceae ATCC 29192]
MAWKIEFDPAAQKELRKLGVQPAKRILKFLSERLALSDNPRSLGAALKGTTLGSLWKYRIGDYRIIVDIEDNVMRILVIR
IGNRKEIYKKSVRH

Nucleotide

• Length: 285 bp

>T25410 NC_015716:23416-23700 [Zymomonas mobilis subsp. pomaceae ATCC 29192]
ATGGCTTGGAAAATTGAATTCGATCCAGCCGCTCAAAAAGAGTTAAGAAAACTAGGGGTACAGCCCGCAAAGCGTATCTT
GAAATTTTTATCTGAACGTTTGGCATTATCGGATAATCCAAGGTCATTAGGCGCTGCTTTAAAAGGGACAACGCTGGGTA
GTTTATGGAAATATCGAATTGGCGATTACCGCATTATTGTCGATATTGAAGATAATGTAATGCGGATTCTGGTTATCAGA
ATTGGTAATAGAAAAGAAATTTATAAAAAATCCGTTAGGCATTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8480.70 Da
• Isoelectric Point: 4.5173

>AT25410 WP_013945642.1 NC_015716:23208-23432 [Zymomonas mobilis subsp. pomaceae ATCC 29192]
MLAIRLPEEIEDRLSELAKATGRTKTFYAREAILQHLEELEDIYLAEARAIKIRSGETKTISLDNVMVEYGLEN

Nucleotide

• Length: 225 bp

>AT25410 NC_015716:23208-23432 [Zymomonas mobilis subsp. pomaceae ATCC 29192]
ATGTTGGCAATTCGTTTACCAGAAGAAATTGAAGATCGTCTAAGCGAACTGGCGAAGGCCACGGGGCGGACAAAAACTTT
TTATGCAAGAGAAGCTATTCTTCAGCATCTAGAAGAACTGGAAGATATCTATCTTGCCGAGGCACGTGCTATCAAAATTC
GTTCCGGAGAAACCAAAACCATTTCTTTAGATAACGTCATGGTTGAATATGGCTTGGAAAATTGA

Structures

Toxin

• AlphaFold DB: F8EWJ3

Antitoxin

• AlphaFold DB: F8EWJ2