Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 486763..487328 Replicon chromosome
Accession NZ_CP102716
Organism Lacticaseibacillus rhamnosus strain DM065

Toxin (Protein)


Gene name mazF Uniprot ID C2JYM4
Locus tag NHB23_RS02270 Protein ID WP_005692155.1
Coordinates 486981..487328 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag NHB23_RS02265 Protein ID WP_020752297.1
Coordinates 486763..486981 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHB23_RS02255 (NHB23_002255) 483057..484847 + 1791 WP_005692150.1 ABC transporter ATP-binding protein -
NHB23_RS02260 (NHB23_002260) 484834..486669 + 1836 WP_015764721.1 ABC transporter ATP-binding protein -
NHB23_RS02265 (NHB23_002265) 486763..486981 + 219 WP_020752297.1 hypothetical protein Antitoxin
NHB23_RS02270 (NHB23_002270) 486981..487328 + 348 WP_005692155.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NHB23_RS02275 (NHB23_002275) 488174..488842 + 669 WP_005692157.1 phenylalanine--tRNA ligase beta subunit-related protein -
NHB23_RS02280 (NHB23_002280) 489083..489901 + 819 WP_014571588.1 ABC transporter permease subunit -
NHB23_RS02285 (NHB23_002285) 489894..490595 + 702 WP_005692159.1 ATP-binding cassette domain-containing protein -
NHB23_RS02290 (NHB23_002290) 490592..491833 + 1242 WP_005692160.1 acyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-90)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13150.01 Da        Isoelectric Point: 7.9817

>T254066 WP_005692155.1 NZ_CP102716:486981-487328 [Lacticaseibacillus rhamnosus]
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF

Download         Length: 348 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8117.33 Da        Isoelectric Point: 5.7995

>AT254066 WP_020752297.1 NZ_CP102716:486763-486981 [Lacticaseibacillus rhamnosus]
MMDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z9DS75

Antitoxin

Source ID Structure

References