Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 389769..390420 Replicon chromosome
Accession NZ_CP102716
Organism Lacticaseibacillus rhamnosus strain DM065

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag NHB23_RS01795 Protein ID WP_005686631.1
Coordinates 390037..390420 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag NHB23_RS01790 Protein ID WP_005686632.1
Coordinates 389769..390017 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NHB23_RS01770 (NHB23_001770) 384818..386206 + 1389 WP_014571607.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
NHB23_RS01775 (NHB23_001775) 386480..387988 + 1509 WP_005686635.1 DEAD/DEAH box helicase -
NHB23_RS01780 (NHB23_001780) 388157..388531 + 375 WP_005686634.1 holo-ACP synthase -
NHB23_RS01785 (NHB23_001785) 388518..389657 + 1140 WP_005691215.1 alanine racemase -
NHB23_RS01790 (NHB23_001790) 389769..390017 + 249 WP_005686632.1 antitoxin Antitoxin
NHB23_RS01795 (NHB23_001795) 390037..390420 + 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NHB23_RS01800 (NHB23_001800) 390934..391461 + 528 WP_005691221.1 QueT transporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T254065 WP_005686631.1 NZ_CP102716:390037-390420 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT254065 WP_005686632.1 NZ_CP102716:389769-390017 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References