Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2686843..2687494 Replicon chromosome
Accession NZ_CP102712
Organism Lacticaseibacillus rhamnosus strain DM054

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag NIM79_RS12500 Protein ID WP_005686631.1
Coordinates 2686843..2687226 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag NIM79_RS12505 Protein ID WP_005686632.1
Coordinates 2687246..2687494 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NIM79_RS12495 (NIM79_012495) 2685879..2686406 - 528 WP_005686630.1 QueT transporter family protein -
NIM79_RS12500 (NIM79_012500) 2686843..2687226 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NIM79_RS12505 (NIM79_012505) 2687246..2687494 - 249 WP_005686632.1 antitoxin Antitoxin
NIM79_RS12510 (NIM79_012510) 2687606..2688745 - 1140 WP_005686633.1 alanine racemase -
NIM79_RS12515 (NIM79_012515) 2688732..2689106 - 375 WP_005686634.1 holo-ACP synthase -
NIM79_RS12520 (NIM79_012520) 2689275..2690783 - 1509 WP_005686635.1 DEAD/DEAH box helicase -
NIM79_RS12525 (NIM79_012525) 2691057..2692445 - 1389 WP_015765066.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T254063 WP_005686631.1 NZ_CP102712:c2687226-2686843 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT254063 WP_005686632.1 NZ_CP102712:c2687494-2687246 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References