Detailed information of TA system TA025406

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 1652765..1653230
• Replicon: chromosome
• Accession: NC_015681
• Organism: Thermodesulfatator indicus DSM 15286

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: F8AAU0
• Locus tag: THEIN_RS08115
• Protein ID: WP_013908193.1
• Coordinates: 1652985..1653230 (+)
• Length: 82 a.a.

Antitoxin (Protein)

• Gene name: hicA
• Uniprot ID: F8AAT9
• Locus tag: THEIN_RS08110
• Protein ID: WP_013908192.1
• Coordinates: 1652765..1652992 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
THEIN_RS08085	1647966..1650179	+	2214	WP_013908188.1	type I DNA topoisomerase	-
THEIN_RS08090	1650359..1651021	+	663	WP_013908189.1	purine or other phosphorylase family 1	-
THEIN_RS08095	1651090..1651263	+	174	WP_013908190.1	twin-arginine translocase TatA/TatE family subunit	-
THEIN_RS08100	1651250..1652581	+	1332	WP_013908191.1	cytochrome c biogenesis protein ResB	-
THEIN_RS08110	1652765..1652992	+	228	WP_013908192.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
THEIN_RS08115	1652985..1653230	+	246	WP_013908193.1	type II toxin-antitoxin system HicA family toxin	Toxin
THEIN_RS08120	1653232..1655145	+	1914	WP_013908194.1	threonine--tRNA ligase	-
THEIN_RS08125	1655154..1655678	+	525	WP_013908195.1	translation initiation factor IF-3	-
THEIN_RS08130	1655710..1655916	+	207	WP_013908196.1	50S ribosomal protein L35	-
THEIN_RS08135	1655932..1656288	+	357	WP_013908197.1	50S ribosomal protein L20	-
THEIN_RS08140	1656290..1657300	+	1011	WP_013908198.1	phenylalanine--tRNA ligase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (12-64)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-51)

Sequences

Toxin

Protein

• Length: 82 a.a.
• Molecular weight: 9273.98 Da
• Isoelectric Point: 11.2210

>T25406 WP_013908193.1 NC_015681:1652985-1653230 [Thermodesulfatator indicus DSM 15286]
MSRLSPVKPELLIKFLESLGFVKARQKGSHIFFRHKDGRTATIPFHKGEEIGRGLLLKILRDVEVSKKDFIKWLSGNKKG
N

Nucleotide

• Length: 246 bp

>T25406 NC_015681:1652985-1653230 [Thermodesulfatator indicus DSM 15286]
ATGTCTAGATTGTCTCCTGTAAAACCTGAATTGTTAATCAAGTTTTTAGAAAGTTTGGGTTTTGTAAAGGCAAGACAAAA
AGGCAGTCACATATTTTTTAGGCATAAAGACGGCCGTACAGCTACCATCCCTTTTCACAAAGGTGAAGAAATAGGTAGGG
GGTTATTGTTAAAAATTTTAAGAGATGTAGAAGTTAGCAAGAAGGATTTTATTAAATGGCTAAGCGGGAATAAAAAGGGG
AATTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8687.98 Da
• Isoelectric Point: 4.1948

>AT25406 WP_013908192.1 NC_015681:1652765-1652992 [Thermodesulfatator indicus DSM 15286]
MKYQFTVLIEKDEDGFYVAEVPELKSCYAQAKTLEEVLKRIKEVVELCIEEQGLPEIQKQFVGLHQVEVEISDNV

Nucleotide

• Length: 228 bp

>AT25406 NC_015681:1652765-1652992 [Thermodesulfatator indicus DSM 15286]
ATGAAATATCAGTTTACAGTTTTAATAGAAAAAGATGAAGACGGCTTTTATGTAGCGGAAGTTCCTGAATTGAAGAGTTG
TTATGCCCAGGCCAAAACACTTGAGGAAGTGCTTAAACGCATAAAAGAAGTTGTTGAATTGTGTATTGAAGAACAAGGGC
TTCCGGAGATCCAGAAACAGTTTGTAGGTCTACATCAGGTTGAAGTAGAAATAAGTGATAATGTCTAG

Structures

Toxin

• AlphaFold DB: F8AAU0

Antitoxin

• AlphaFold DB: F8AAT9