Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 127910..128646 Replicon plasmid pGN4542-1
Accession NZ_CP102693
Organism Klebsiella pneumoniae strain GN4542

Toxin (Protein)


Gene name tacT Uniprot ID A0A486JQ35
Locus tag NV951_RS26360 Protein ID WP_049109282.1
Coordinates 127910..128392 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag NV951_RS26365 Protein ID WP_003026799.1
Coordinates 128380..128646 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NV951_RS26330 (NV951_26325) 123130..124095 + 966 WP_004118146.1 DMT family transporter -
NV951_RS26335 (NV951_26330) 124163..124291 - 129 Protein_135 transcriptional regulator -
NV951_RS26340 (NV951_26335) 124351..124965 - 615 WP_023316428.1 hypothetical protein -
NV951_RS26345 (NV951_26340) 125146..125343 - 198 Protein_137 hypothetical protein -
NV951_RS26350 (NV951_26345) 125646..126278 + 633 WP_001567369.1 hypothetical protein -
NV951_RS26355 (NV951_26350) 126307..127710 - 1404 WP_275622086.1 ISNCY-like element ISKpn21 family transposase -
NV951_RS26360 (NV951_26355) 127910..128392 - 483 WP_049109282.1 GNAT family N-acetyltransferase Toxin
NV951_RS26365 (NV951_26360) 128380..128646 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
NV951_RS26370 (NV951_26365) 128932..129039 + 108 Protein_142 transposase -
NV951_RS26375 (NV951_26370) 129082..129897 - 816 WP_049109278.1 hypothetical protein -
NV951_RS26380 (NV951_26375) 130185..131210 + 1026 WP_064782720.1 IS110 family transposase -
NV951_RS26385 (NV951_26380) 131549..132472 + 924 WP_049108798.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid dfrA1 / qacE / sul1 / qnrB4 / blaDHA-1 - 1..178593 178593
- inside IScluster/Tn - - 126307..139566 13259


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(44-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17336.86 Da        Isoelectric Point: 8.0020

>T254031 WP_049109282.1 NZ_CP102693:c128392-127910 [Klebsiella pneumoniae]
VGRITAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCETGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT254031 WP_003026799.1 NZ_CP102693:c128646-128380 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486JQ35

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References