Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5186854..5187479 Replicon chromosome
Accession NZ_CP102692
Organism Klebsiella pneumoniae strain GN4542

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NV951_RS25295 Protein ID WP_002882817.1
Coordinates 5186854..5187237 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NV951_RS25300 Protein ID WP_004150355.1
Coordinates 5187237..5187479 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NV951_RS25280 (NV951_25275) 5184220..5185122 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NV951_RS25285 (NV951_25280) 5185119..5185754 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NV951_RS25290 (NV951_25285) 5185751..5186680 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NV951_RS25295 (NV951_25290) 5186854..5187237 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NV951_RS25300 (NV951_25295) 5187237..5187479 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NV951_RS25305 (NV951_25300) 5187684..5188601 + 918 WP_049109136.1 alpha/beta hydrolase -
NV951_RS25310 (NV951_25305) 5188615..5189556 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NV951_RS25315 (NV951_25310) 5189601..5190038 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NV951_RS25320 (NV951_25315) 5190035..5190895 - 861 WP_002882807.1 virulence factor BrkB family protein -
NV951_RS25325 (NV951_25320) 5190889..5191488 - 600 WP_004146231.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T254028 WP_002882817.1 NZ_CP102692:c5187237-5186854 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT254028 WP_004150355.1 NZ_CP102692:c5187479-5187237 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References