Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4597307..4598117 Replicon chromosome
Accession NZ_CP102692
Organism Klebsiella pneumoniae strain GN4542

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag NV951_RS22525 Protein ID WP_004178461.1
Coordinates 4597307..4597840 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NV951_RS22530 Protein ID WP_002887278.1
Coordinates 4597851..4598117 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NV951_RS22520 (NV951_22515) 4596138..4597259 + 1122 WP_004214138.1 cupin domain-containing protein -
NV951_RS22525 (NV951_22520) 4597307..4597840 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
NV951_RS22530 (NV951_22525) 4597851..4598117 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NV951_RS22535 (NV951_22530) 4598220..4599653 - 1434 WP_016946714.1 Cu(+)/Ag(+) sensor histidine kinase -
NV951_RS22540 (NV951_22535) 4599643..4600326 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NV951_RS22545 (NV951_22540) 4600498..4601880 + 1383 WP_040165580.1 efflux transporter outer membrane subunit -
NV951_RS22550 (NV951_22545) 4601898..4602242 + 345 WP_040148409.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T254025 WP_004178461.1 NZ_CP102692:c4597840-4597307 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT254025 WP_002887278.1 NZ_CP102692:c4598117-4597851 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References