Detailed information of TA system TA025401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 2551439..2552118
• Replicon: chromosome
• Accession: NC_015677
• Organism: Ramlibacter tataouinensis TTB310

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F5Y199
• Locus tag: RTA_RS12295
• Protein ID: WP_013901732.1
• Coordinates: 2551439..2551864 (-)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F5Y1A0
• Locus tag: RTA_RS12300
• Protein ID: WP_013901733.1
• Coordinates: 2551864..2552118 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RTA_RS12265	2546504..2547496	+	993	WP_049871277.1	Bug family tripartite tricarboxylate transporter substrate binding protein	-
RTA_RS12270	2547508..2547987	-	480	WP_013901727.1	molybdopterin synthase catalytic subunit MoaE	-
RTA_RS12275	2547993..2548244	-	252	WP_013901728.1	MoaD/ThiS family protein	-
RTA_RS12280	2548241..2549479	-	1239	WP_013901729.1	molybdopterin molybdotransferase MoeA	-
RTA_RS12285	2549476..2550009	-	534	WP_013901730.1	molybdopterin-guanine dinucleotide biosynthesis protein B	-
RTA_RS12290	2550006..2551427	-	1422	WP_013901731.1	threonine synthase	-
RTA_RS12295	2551439..2551864	-	426	WP_013901732.1	type II toxin-antitoxin system VapC family toxin	Toxin
RTA_RS12300	2551864..2552118	-	255	WP_013901733.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RTA_RS12305	2552190..2553524	-	1335	WP_013901734.1	homoserine dehydrogenase	-
RTA_RS12310	2553521..2554747	-	1227	WP_013901735.1	pyridoxal phosphate-dependent aminotransferase	-
RTA_RS12315	2554811..2555188	+	378	WP_041675474.1	Mth938-like domain-containing protein	-
RTA_RS12320	2555300..2555776	+	477	WP_013901737.1	peroxiredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-126)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-50)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15900.31 Da
• Isoelectric Point: 9.9022

>T25401 WP_013901732.1 NC_015677:c2551864-2551439 [Ramlibacter tataouinensis TTB310]
MLWLLDTCVVSEALRRQADAAVVKWLEAHTARAALPVIALGEIQYGIERLEPGRRRNALQAWRDTLQARYADRTLDVGEP
ILRVWARLRASLDAIGRPQQDMDILIAATALAHNLTLVTRNTRHFADTGVRLFDPWTGKHA

Nucleotide

• Length: 426 bp

>T25401 NC_015677:c2551864-2551439 [Ramlibacter tataouinensis TTB310]
ATGCTCTGGTTGCTTGACACATGCGTGGTTTCGGAAGCGCTCAGGCGCCAAGCCGACGCGGCGGTGGTGAAGTGGTTGGA
AGCGCATACCGCGCGGGCCGCGTTGCCCGTCATCGCGCTTGGCGAAATCCAGTACGGCATCGAGCGCCTCGAGCCCGGGC
GGCGGCGCAACGCGCTGCAGGCATGGCGTGACACCTTGCAAGCCCGCTATGCAGACCGGACGTTGGATGTCGGCGAGCCG
ATCCTGCGAGTCTGGGCCCGCCTGCGTGCCTCGCTTGACGCCATCGGACGGCCGCAACAGGACATGGACATCCTCATCGC
CGCCACAGCCCTGGCCCACAATCTAACCCTGGTCACCCGCAACACCCGCCACTTCGCCGACACCGGTGTGCGCCTGTTCG
ATCCCTGGACCGGCAAGCACGCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9285.63 Da
• Isoelectric Point: 6.7260

>AT25401 WP_013901733.1 NC_015677:c2552118-2551864 [Ramlibacter tataouinensis TTB310]
MAATWQVHDAKNRLSELMDRAVKEGPQVITRHGRPVVKVVAVDAHEATQPAGDDGFFEFLMSAPKIDELELPVRRGRKAP
PELG

Nucleotide

• Length: 255 bp

>AT25401 NC_015677:c2552118-2551864 [Ramlibacter tataouinensis TTB310]
ATGGCTGCAACCTGGCAAGTGCACGACGCCAAGAACCGTCTGAGCGAGTTGATGGATCGCGCCGTCAAAGAAGGGCCGCA
GGTCATCACGCGCCATGGCCGGCCGGTGGTCAAGGTGGTGGCCGTGGACGCGCATGAGGCCACGCAGCCGGCCGGGGACG
ATGGTTTCTTCGAGTTCCTGATGAGTGCGCCCAAGATCGACGAGCTTGAACTGCCGGTCCGGCGGGGGCGCAAGGCACCG
CCGGAACTCGGCTGA

Structures

Toxin

• AlphaFold DB: F5Y199

Antitoxin

• AlphaFold DB: F5Y1A0