Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3445916..3446541 Replicon chromosome
Accession NZ_CP102687
Organism Klebsiella pneumoniae strain GN4539

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NV950_RS16905 Protein ID WP_002882817.1
Coordinates 3445916..3446299 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NV950_RS16910 Protein ID WP_004150355.1
Coordinates 3446299..3446541 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NV950_RS16890 (NV950_16885) 3443282..3444184 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NV950_RS16895 (NV950_16890) 3444181..3444816 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NV950_RS16900 (NV950_16895) 3444813..3445742 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NV950_RS16905 (NV950_16900) 3445916..3446299 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NV950_RS16910 (NV950_16905) 3446299..3446541 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NV950_RS16915 (NV950_16910) 3446746..3447663 + 918 WP_002882812.1 alpha/beta hydrolase -
NV950_RS16920 (NV950_16915) 3447677..3448618 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NV950_RS16925 (NV950_16920) 3448663..3449100 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NV950_RS16930 (NV950_16925) 3449097..3449957 - 861 WP_002882807.1 virulence factor BrkB family protein -
NV950_RS16935 (NV950_16930) 3449951..3450550 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T254009 WP_002882817.1 NZ_CP102687:c3446299-3445916 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT254009 WP_004150355.1 NZ_CP102687:c3446541-3446299 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References