Detailed information of TA system TA025400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1912401..1913068
• Replicon: chromosome
• Accession: NC_015677
• Organism: Ramlibacter tataouinensis TTB310

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F5XWF7
• Locus tag: RTA_RS09335
• Protein ID: WP_013901143.1
• Coordinates: 1912631..1913068 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F5XWF6
• Locus tag: RTA_RS09330
• Protein ID: WP_013901142.1
• Coordinates: 1912401..1912634 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RTA_RS09305	1907642..1908499	+	858	WP_013901137.1	enoyl-CoA hydratase family protein	-
RTA_RS09310	1908501..1909670	+	1170	WP_013901138.1	acyl-CoA dehydrogenase family protein	-
RTA_RS09315	1909667..1910062	+	396	WP_013901139.1	RidA family protein	-
RTA_RS09320	1910073..1911251	+	1179	WP_013901140.1	acyl-CoA dehydrogenase	-
RTA_RS09325	1911370..1912326	+	957	WP_143763056.1	tripartite tricarboxylate transporter substrate binding protein	-
RTA_RS09330	1912401..1912634	+	234	WP_013901142.1	antitoxin	Antitoxin
RTA_RS09335	1912631..1913068	+	438	WP_013901143.1	type II toxin-antitoxin system VapC family toxin	Toxin
RTA_RS09340	1913071..1913706	-	636	WP_013901144.1	GntR family transcriptional regulator	-
RTA_RS09345	1913787..1915940	+	2154	WP_013901145.1	methylmalonyl-CoA mutase	-
RTA_RS09350	1915937..1916935	+	999	WP_013901146.1	methylmalonyl Co-A mutase-associated GTPase MeaB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15101.23 Da
• Isoelectric Point: 4.4870

>T25400 WP_013901143.1 NC_015677:1912631-1913068 [Ramlibacter tataouinensis TTB310]
MIILDTNVVSEPLKPQPEPAVLRWLDAQDPQTLYLTAVNLAELLAGVGALAAGPRRAGLERAFDTQVLPLFDGRILAFDE
PAARSFARVAASAQAAGNPIGFADAAVAAIGAANGFLIAARNVRDFRGTGVDVINPWEAEAAKPG

Nucleotide

• Length: 438 bp

>T25400 NC_015677:1912631-1913068 [Ramlibacter tataouinensis TTB310]
ATGATCATTCTCGATACGAACGTCGTTTCGGAACCGCTCAAGCCCCAGCCCGAGCCAGCCGTTTTGAGGTGGCTGGACGC
GCAGGATCCACAAACGCTGTATCTCACGGCGGTCAACCTGGCCGAGCTGCTGGCTGGTGTTGGAGCGCTGGCGGCAGGCC
CTAGGCGGGCTGGGTTGGAACGGGCATTCGATACGCAGGTGCTGCCGCTGTTCGACGGGCGCATCCTGGCTTTCGATGAA
CCAGCGGCGCGATCCTTTGCCCGCGTGGCTGCTTCGGCCCAGGCTGCCGGCAACCCCATCGGTTTCGCCGACGCAGCCGT
TGCCGCCATCGGTGCGGCGAATGGCTTCCTGATCGCGGCGCGCAATGTGCGCGACTTCCGCGGTACCGGTGTGGACGTGA
TCAATCCGTGGGAAGCCGAGGCAGCCAAACCGGGTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8337.65 Da
• Isoelectric Point: 11.8858

>AT25400 WP_013901142.1 NC_015677:1912401-1912634 [Ramlibacter tataouinensis TTB310]
MPAVTVRNLTEATHRALKSRAARHGRSTEAEIRAILEESVRPRVGLGSQLLAVGRRLGGVELPLVRDKTPVAPATFE

Nucleotide

• Length: 234 bp

>AT25400 NC_015677:1912401-1912634 [Ramlibacter tataouinensis TTB310]
ATGCCTGCCGTCACTGTTCGCAATCTCACTGAAGCGACCCATCGCGCACTCAAGAGCCGTGCCGCTCGACATGGGCGAAG
CACCGAAGCCGAGATCCGGGCCATCCTGGAAGAGTCGGTGCGCCCCCGGGTGGGCCTGGGCAGCCAGTTGCTGGCCGTGG
GTCGCCGTCTGGGCGGTGTCGAGTTGCCGCTGGTGCGCGACAAGACGCCGGTGGCGCCGGCGACTTTCGAATGA

Structures

Toxin

• AlphaFold DB: F5XWF7

Antitoxin

• AlphaFold DB: F5XWF6