Detailed information of TA system TA025399

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafQ-relB/YafQ-RelB
• Location: 5891578..5892107
• Replicon: chromosome
• Accession: NC_015675
• Organism: Mesorhizobium opportunistum WSM2075

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: F7YE00
• Locus tag: MESOP_RS28425
• Protein ID: WP_013896799.1
• Coordinates: 5891838..5892107 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F7YDZ9
• Locus tag: MESOP_RS28420
• Protein ID: WP_013896798.1
• Coordinates: 5891578..5891835 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MESOP_RS28405	5886585..5888225	-	1641	WP_013896795.1	acyl-CoA synthetase	-
MESOP_RS28410	5888412..5889377	+	966	WP_013896796.1	sugar-binding transcriptional regulator	-
MESOP_RS28415	5889466..5891520	+	2055	WP_013896797.1	bifunctional aldolase/short-chain dehydrogenase	-
MESOP_RS28420	5891578..5891835	+	258	WP_013896798.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MESOP_RS28425	5891838..5892107	+	270	WP_013896799.1	type II toxin-antitoxin system YafQ family toxin	Toxin
MESOP_RS28430	5892185..5893237	-	1053	WP_013896800.1	substrate-binding domain-containing protein	-
MESOP_RS28435	5893287..5894282	-	996	WP_013896801.1	ABC transporter permease	-
MESOP_RS28440	5894365..5895876	-	1512	WP_013896802.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (7-88)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-83)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10179.71 Da
• Isoelectric Point: 9.6561

>T25399 WP_013896799.1 NC_015675:5891838-5892107 [Mesorhizobium opportunistum WSM2075]
MPAAIHSGQFRRDVKRMEKRGKNLGKLRDLLGLLIAGHELPATYKDPLKGDWKGFREGHIEPDWLLIYRVVGDELQLART
GSHSDLFNE

Nucleotide

• Length: 270 bp

>T25399 NC_015675:5891838-5892107 [Mesorhizobium opportunistum WSM2075]
ATGCCAGCGGCCATTCACTCCGGGCAATTTCGCCGGGATGTGAAGCGTATGGAAAAGCGTGGCAAGAACCTTGGGAAATT
GCGCGATCTGCTAGGCTTGCTGATCGCCGGGCATGAACTGCCGGCGACGTATAAGGATCCTCTGAAAGGCGACTGGAAAG
GCTTTCGAGAGGGGCATATCGAACCGGACTGGCTCCTGATCTACCGCGTTGTCGGCGACGAATTGCAGCTTGCCCGTACC
GGCTCGCACTCCGACCTATTCAACGAGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9147.63 Da
• Isoelectric Point: 8.6840

>AT25399 WP_013896798.1 NC_015675:5891578-5891835 [Mesorhizobium opportunistum WSM2075]
MATDAVVRARIDAATKDQATEALAAMGLSVSDAIRLLLVRVAADKEFPFPVKVPNATTRKAMAELEKGKDKRFASADELF
KDLGI

Nucleotide

• Length: 258 bp

>AT25399 NC_015675:5891578-5891835 [Mesorhizobium opportunistum WSM2075]
ATGGCTACAGATGCAGTGGTCCGCGCTCGGATCGACGCCGCGACCAAGGATCAGGCGACGGAAGCCTTGGCGGCCATGGG
CCTGTCGGTTTCCGACGCTATCCGCCTGCTGCTGGTCCGCGTGGCCGCAGACAAGGAATTTCCCTTCCCGGTGAAAGTGC
CCAACGCAACCACGCGAAAGGCTATGGCTGAGTTGGAAAAAGGCAAAGACAAGCGCTTCGCCTCGGCTGATGAATTGTTC
AAGGATCTGGGGATCTAA

Structures

Toxin

• AlphaFold DB: F7YE00

Antitoxin

• AlphaFold DB: F7YDZ9