Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3856007..3856583 Replicon chromosome
Accession NZ_CP102679
Organism Escherichia coli strain XH989

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag NU949_RS20980 Protein ID WP_001131965.1
Coordinates 3856296..3856583 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag NU949_RS20975 Protein ID WP_019842999.1
Coordinates 3856007..3856309 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NU949_RS20955 (3851620) 3851620..3852132 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
NU949_RS20960 (3852522) 3852522..3854672 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
NU949_RS20965 (3854803) 3854803..3855006 + 204 WP_000467859.1 YbdD/YjiX family protein -
NU949_RS20970 (3855017) 3855017..3855973 + 957 WP_001295525.1 GTPase -
NU949_RS20975 (3856007) 3856007..3856309 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
NU949_RS20980 (3856296) 3856296..3856583 - 288 WP_001131965.1 BrnT family toxin Toxin
NU949_RS20985 (3856780) 3856780..3857946 + 1167 WP_000800825.1 restriction endonuclease -
NU949_RS20990 (3858010) 3858010..3859629 + 1620 WP_001029743.1 class I SAM-dependent DNA methyltransferase -
NU949_RS20995 (3859619) 3859619..3861043 + 1425 WP_000535015.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaCTX-M-55 cheD / fimH / fimG / fimF / fimD / fimC / fimI / fimA / fimE / fimB 3769809..3941514 171705


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T253988 WP_001131965.1 NZ_CP102679:c3856583-3856296 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT253988 WP_019842999.1 NZ_CP102679:c3856309-3856007 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References