Detailed information of TA system TA025397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 5034811..5035346
• Replicon: chromosome
• Accession: NC_015675
• Organism: Mesorhizobium opportunistum WSM2075

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F7Y055
• Locus tag: MESOP_RS24380
• Protein ID: WP_013895991.1
• Coordinates: 5034811..5035086 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F7Y056
• Locus tag: MESOP_RS24385
• Protein ID: WP_013895992.1
• Coordinates: 5035086..5035346 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MESOP_RS24355	5030829..5031740	-	912	WP_013895986.1	ABC transporter substrate-binding protein	-
MESOP_RS24360	5031948..5032520	+	573	WP_013895987.1	NAD(P)H-dependent oxidoreductase	-
MESOP_RS24365	5032530..5033741	-	1212	WP_013895988.1	MFS transporter	-
MESOP_RS24370	5033738..5034238	-	501	WP_013895989.1	transcription antitermination factor NusB	-
MESOP_RS24375	5034235..5034729	-	495	WP_013895990.1	6,7-dimethyl-8-ribityllumazine synthase	-
MESOP_RS24380	5034811..5035086	-	276	WP_013895991.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MESOP_RS24385	5035086..5035346	-	261	WP_013895992.1	ribbon-helix-helix domain-containing protein	Antitoxin
MESOP_RS24390	5035416..5036033	-	618	WP_013895993.1	riboflavin synthase	-
MESOP_RS24395	5036219..5037349	-	1131	WP_013895994.1	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	-
MESOP_RS24400	5037352..5037831	-	480	WP_013895995.1	transcriptional regulator NrdR	-
MESOP_RS24405	5037989..5038567	-	579	WP_013895996.1	hypothetical protein	-
MESOP_RS24410	5038707..5040020	-	1314	WP_013895997.1	serine hydroxymethyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10496.05 Da
• Isoelectric Point: 8.5620

>T25397 WP_013895991.1 NC_015675:c5035086-5034811 [Mesorhizobium opportunistum WSM2075]
MELKWTNKALSDLVRLHDFLAPANGPAAARAVQALAAAPARLVEYPRIGERLEEFEPREVRRILVGSYEIRYEIQNATIS
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T25397 NC_015675:c5035086-5034811 [Mesorhizobium opportunistum WSM2075]
ATGGAGCTGAAATGGACCAATAAGGCTTTGTCCGACTTGGTCCGGCTCCACGATTTTCTCGCGCCAGCAAATGGCCCGGC
GGCCGCACGCGCCGTGCAGGCGCTTGCTGCCGCGCCGGCACGGTTAGTCGAGTATCCGCGCATCGGTGAGAGACTAGAAG
AGTTCGAGCCACGAGAAGTGCGCCGCATTTTGGTAGGCAGCTACGAAATTCGTTACGAAATACAGAACGCGACAATTTCT
GTGTTGCGCCTGTGGCATACGCGAGAGGATCGATAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9614.05 Da
• Isoelectric Point: 5.6921

>AT25397 WP_013895992.1 NC_015675:c5035346-5035086 [Mesorhizobium opportunistum WSM2075]
MDTKVITAHVPLPLAEKVDQLAARLDRPRGWIVKQALSAWVDLEEERRRLTLEALADVDAGRVIDHQAVQAWAESLGTDK
PLPVPR

Nucleotide

• Length: 261 bp

>AT25397 NC_015675:c5035346-5035086 [Mesorhizobium opportunistum WSM2075]
ATGGACACAAAAGTAATCACCGCTCACGTTCCGCTGCCGCTAGCAGAAAAGGTGGATCAGCTAGCTGCTCGCCTCGATCG
CCCGCGCGGCTGGATCGTCAAACAAGCTCTTTCCGCGTGGGTGGATTTGGAGGAGGAGCGCCGACGTCTCACGCTTGAAG
CACTGGCCGATGTGGATGCAGGACGGGTCATCGATCACCAGGCCGTGCAGGCGTGGGCTGAGAGTCTTGGCACCGACAAG
CCACTACCGGTGCCTCGTTGA

Structures

Toxin

• AlphaFold DB: F7Y055

Antitoxin

• AlphaFold DB: F7Y056