Detailed information of TA system TA025396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3236250..3237031
• Replicon: chromosome
• Accession: NC_015675
• Organism: Mesorhizobium opportunistum WSM2075

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: F7Y9D1
• Locus tag: MESOP_RS15535
• Protein ID: WP_013894267.1
• Coordinates: 3236250..3236744 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: F7Y9D2
• Locus tag: MESOP_RS15540
• Protein ID: WP_013894268.1
• Coordinates: 3236741..3237031 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MESOP_RS15505	3231430..3232218	-	789	WP_013894262.1	ABC transporter substrate-binding protein	-
MESOP_RS15510	3232329..3233489	-	1161	WP_013894263.1	saccharopine dehydrogenase family protein	-
MESOP_RS15515	3233486..3233986	-	501	WP_013894264.1	Lrp/AsnC family transcriptional regulator	-
MESOP_RS15520	3234150..3235022	-	873	WP_013894265.1	LysR family transcriptional regulator	-
MESOP_RS15530	3235371..3236120	+	750	WP_013894266.1	sulfite exporter TauE/SafE family protein	-
MESOP_RS15535	3236250..3236744	-	495	WP_013894267.1	GNAT family N-acetyltransferase	Toxin
MESOP_RS15540	3236741..3237031	-	291	WP_013894268.1	DUF1778 domain-containing protein	Antitoxin
MESOP_RS15545	3237130..3237579	-	450	WP_013894269.1	universal stress protein	-
MESOP_RS15550	3237844..3238425	+	582	WP_013894270.1	histidine phosphatase family protein	-
MESOP_RS15555	3238702..3239103	-	402	WP_013894271.1	VOC family protein	-
MESOP_RS15560	3239201..3240253	-	1053	WP_013894272.1	sigma-70 family RNA polymerase sigma factor	-
MESOP_RS15565	3240296..3241042	-	747	WP_013894273.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17380.98 Da
• Isoelectric Point: 8.9644

>T25396 WP_013894267.1 NC_015675:c3236744-3236250 [Mesorhizobium opportunistum WSM2075]
VSLSAPVPLADHHDLNDFSSGIPSLDDWLKKRARANQAGGASRTYVTCIGNRVVGYYAIASGGVRMVEATGRFRRNMPDP
IPVVVLGRLAVDQSHHGRGIGRALVRDAGLRILQAAEILGIRGILVHAISDDARAFYMAVGFEPSPVEPLTLMATLADLG
ASLG

Nucleotide

• Length: 495 bp

>T25396 NC_015675:c3236744-3236250 [Mesorhizobium opportunistum WSM2075]
GTGAGCCTGTCGGCCCCGGTTCCGCTCGCCGACCATCACGACCTCAATGATTTTTCATCGGGCATACCCTCACTCGACGA
CTGGCTGAAAAAACGGGCGCGCGCCAACCAGGCAGGCGGTGCATCCCGGACCTATGTGACCTGCATCGGCAACCGTGTCG
TTGGCTATTACGCTATTGCCAGCGGAGGAGTGCGGATGGTCGAAGCTACCGGCCGCTTCCGGCGCAACATGCCCGACCCG
ATCCCGGTTGTGGTGCTCGGCAGGTTGGCGGTCGACCAGAGCCATCACGGCCGTGGCATCGGCCGTGCGCTGGTAAGGGA
TGCCGGTCTGCGGATACTTCAGGCCGCTGAAATACTGGGAATCAGGGGTATACTGGTGCATGCGATATCCGACGATGCCC
GAGCCTTCTATATGGCTGTCGGATTCGAGCCTTCACCAGTCGAGCCGCTGACGCTCATGGCCACGCTCGCCGACCTCGGC
GCGAGTCTCGGTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10762.39 Da
• Isoelectric Point: 9.9898

>AT25396 WP_013894268.1 NC_015675:c3237031-3236741 [Mesorhizobium opportunistum WSM2075]
MPASHQPLPKSVPLNIRVKPEVRNLIDRAAELLGKNRTDFMLEASQRAAEDALLNRLVFTAEPDAYAAFLARLDMSPHPN
DRLRRTMATKAPWDAA

Nucleotide

• Length: 291 bp

>AT25396 NC_015675:c3237031-3236741 [Mesorhizobium opportunistum WSM2075]
ATGCCAGCCTCACATCAGCCGTTGCCGAAGAGCGTTCCGCTCAATATCCGCGTCAAGCCAGAGGTCCGCAATCTGATCGA
CAGGGCAGCGGAACTGCTTGGCAAGAACCGCACGGACTTCATGCTGGAGGCGTCGCAACGCGCCGCCGAGGACGCGCTGC
TCAACCGTCTCGTGTTTACTGCCGAGCCGGATGCCTACGCTGCGTTTCTGGCGCGTCTCGACATGTCGCCGCATCCGAAC
GACCGGTTGCGGCGTACGATGGCAACAAAGGCTCCGTGGGACGCTGCGTGA

Structures

Toxin

• AlphaFold DB: F7Y9D1

Antitoxin

• AlphaFold DB: F7Y9D2