Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2020604..2021180 Replicon chromosome
Accession NZ_CP102675
Organism Escherichia coli strain XH987

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag NU950_RS11780 Protein ID WP_001131965.1
Coordinates 2020893..2021180 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag NU950_RS11775 Protein ID WP_019842999.1
Coordinates 2020604..2020906 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NU950_RS11755 (2016217) 2016217..2016729 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
NU950_RS11760 (2017119) 2017119..2019269 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
NU950_RS11765 (2019400) 2019400..2019603 + 204 WP_000467859.1 YbdD/YjiX family protein -
NU950_RS11770 (2019614) 2019614..2020570 + 957 WP_001295525.1 GTPase -
NU950_RS11775 (2020604) 2020604..2020906 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
NU950_RS11780 (2020893) 2020893..2021180 - 288 WP_001131965.1 BrnT family toxin Toxin
NU950_RS11785 (2021377) 2021377..2022543 + 1167 WP_000800825.1 restriction endonuclease -
NU950_RS11790 (2022607) 2022607..2024226 + 1620 WP_001029743.1 class I SAM-dependent DNA methyltransferase -
NU950_RS11795 (2024216) 2024216..2025640 + 1425 WP_000535015.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaCTX-M-55 cheD / fimH / fimG / fimF / fimD / fimC / fimI / fimA / fimE / fimB 1934407..2106111 171704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T253943 WP_001131965.1 NZ_CP102675:c2021180-2020893 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT253943 WP_019842999.1 NZ_CP102675:c2020906-2020604 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References