Detailed information of TA system TA025394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2564515..2565167
• Replicon: chromosome
• Accession: NC_015675
• Organism: Mesorhizobium opportunistum WSM2075

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F7YH07
• Locus tag: MESOP_RS12350
• Protein ID: WP_013893670.1
• Coordinates: 2564515..2564904 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7YH08
• Locus tag: MESOP_RS12355
• Protein ID: WP_013893671.1
• Coordinates: 2564901..2565167 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MESOP_RS12320	2559867..2560682	+	816	WP_013893664.1	oxidoreductase	-
MESOP_RS12325	2560729..2561322	-	594	WP_013893665.1	helix-turn-helix transcriptional regulator	-
MESOP_RS12330	2561388..2561813	+	426	WP_013893666.1	VOC family protein	-
MESOP_RS12335	2561819..2562769	-	951	WP_013893667.1	trypsin-like peptidase domain-containing protein	-
MESOP_RS12340	2562995..2563867	+	873	WP_013893668.1	S1C family serine protease	-
MESOP_RS32960	2563876..2564508	+	633	WP_013893669.1	helix-turn-helix transcriptional regulator	-
MESOP_RS12350	2564515..2564904	-	390	WP_013893670.1	type II toxin-antitoxin system VapC family toxin	Toxin
MESOP_RS12355	2564901..2565167	-	267	WP_013893671.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MESOP_RS12360	2565334..2566200	+	867	WP_013893672.1	NAD(P)-dependent oxidoreductase	-
MESOP_RS12365	2566197..2566490	-	294	WP_013893673.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MESOP_RS12370	2566487..2566723	-	237	WP_013893674.1	type II toxin-antitoxin system ParD family antitoxin	-
MESOP_RS12375	2567184..2569430	-	2247	WP_013893675.1	OmpA family protein	-
MESOP_RS12380	2569628..2570074	+	447	WP_041164638.1	YcgN family cysteine cluster protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-123)

Antitoxin

• Antitoxin VapB-like (1-86)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14273.51 Da
• Isoelectric Point: 5.6319

>T25394 WP_013893670.1 NC_015675:c2564904-2564515 [Mesorhizobium opportunistum WSM2075]
MIIDSSVLVAILRLEPAYERFVLAITQANRRLLPAPTFLETTMVLAGGRQDEILDRLDSFLHTASIETVPFTADHAAVAR
QAFLRYGKGRHPAALNFGDCIAYATARLEAMPLLFKGDDFRLTDIEPAV

Nucleotide

• Length: 390 bp

>T25394 NC_015675:c2564904-2564515 [Mesorhizobium opportunistum WSM2075]
ATGATCATCGACTCCTCGGTCCTGGTCGCCATTCTGCGTCTCGAGCCAGCGTATGAACGTTTCGTGCTCGCGATCACGCA
GGCCAACAGACGGCTGTTGCCGGCCCCAACTTTTCTCGAAACGACCATGGTACTTGCGGGCGGCCGGCAAGATGAAATCC
TGGATCGGCTCGACAGCTTCCTGCACACCGCCTCGATCGAAACAGTCCCGTTCACAGCTGACCATGCGGCTGTCGCCCGC
CAGGCATTCCTCCGCTATGGCAAGGGCCGGCATCCGGCTGCGCTGAACTTCGGCGACTGCATCGCCTACGCCACAGCGCG
GCTCGAAGCCATGCCGTTGCTTTTCAAGGGTGATGACTTCCGGCTGACCGACATCGAACCGGCGGTCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10174.60 Da
• Isoelectric Point: 5.2709

>AT25394 WP_013893671.1 NC_015675:c2565167-2564901 [Mesorhizobium opportunistum WSM2075]
MALSIKNMEVEQLARELARRRRVSVTEAIRQSLEREVARERLVPREETSDLYQRLLAISESAGKIPKRENAMTDDEILGY
DEFGIPTK

Nucleotide

• Length: 267 bp

>AT25394 NC_015675:c2565167-2564901 [Mesorhizobium opportunistum WSM2075]
ATGGCGCTTTCGATCAAGAACATGGAAGTCGAGCAACTGGCGCGCGAACTCGCCCGCCGCCGTCGCGTGTCTGTGACAGA
GGCTATTCGTCAGAGCCTCGAGCGTGAAGTGGCGCGCGAACGGCTGGTGCCGCGTGAAGAAACCAGCGACCTGTATCAGA
GATTGCTTGCGATTTCCGAAAGCGCCGGAAAAATCCCAAAACGCGAAAATGCGATGACTGACGACGAAATCCTCGGTTAC
GACGAGTTTGGAATCCCGACGAAATGA

Structures

Toxin

• AlphaFold DB: F7YH07

Antitoxin

• AlphaFold DB: F7YH08