Detailed information of TA system TA025393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): brnT-shpB/BrnT-BrnA
• Location: 1334200..1334755
• Replicon: chromosome
• Accession: NC_015675
• Organism: Mesorhizobium opportunistum WSM2075

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: MESOP_RS33390
• Protein ID: WP_083833218.1
• Coordinates: 1334200..1334487 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: F7YFM1
• Locus tag: MESOP_RS06345
• Protein ID: WP_013892502.1
• Coordinates: 1334468..1334755 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MESOP_RS06320	1330565..1331404	-	840	WP_013892497.1	MoxR family ATPase	-
MESOP_RS06325	1331800..1332387	+	588	WP_013892498.1	5-formyltetrahydrofolate cyclo-ligase	-
MESOP_RS06330	1332459..1333283	+	825	WP_013892499.1	YmdB family metallophosphoesterase	-
MESOP_RS06335	1333354..1334121	+	768	WP_013892500.1	TerC family protein	-
MESOP_RS33390	1334200..1334487	+	288	WP_083833218.1	BrnT family toxin	Toxin
MESOP_RS06345	1334468..1334755	+	288	WP_013892502.1	BrnA antitoxin family protein	Antitoxin
MESOP_RS06350	1334752..1335228	-	477	WP_013892503.1	SRPBCC family protein	-
MESOP_RS06355	1335377..1335769	-	393	WP_013892504.1	VOC family protein	-
MESOP_RS06360	1335794..1336276	-	483	WP_013892505.1	SRPBCC domain-containing protein	-
MESOP_RS06365	1336328..1336651	+	324	WP_013892506.1	helix-turn-helix domain-containing protein	-
MESOP_RS06370	1336855..1337604	+	750	WP_013892507.1	YebC/PmpR family DNA-binding transcriptional regulator	-
MESOP_RS06375	1337601..1337918	+	318	WP_013892508.1	SelT/SelW/SelH family protein	-
MESOP_RS33395	1337937..1338086	-	150	WP_083833219.1	hypothetical protein	-
MESOP_RS06380	1338144..1338623	-	480	WP_013892509.1	DUF1772 domain-containing protein	-
MESOP_RS06385	1338734..1339375	+	642	WP_013892510.1	TetR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-86)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (29-86)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11187.67 Da
• Isoelectric Point: 6.3336

>T25393 WP_083833218.1 NC_015675:1334200-1334487 [Mesorhizobium opportunistum WSM2075]
MQFEWDFEKARRNLAKHGIAFDLAQKVFADPLHLIVPDRFEDGEQRWHAIGAIGAVVVLLVVHTYPDREDEERIRIVGAR
KATPHERRRYEQEEP

Nucleotide

• Length: 288 bp

>T25393 NC_015675:1334200-1334487 [Mesorhizobium opportunistum WSM2075]
TTGCAGTTCGAATGGGACTTTGAAAAAGCCAGGCGCAACCTTGCCAAGCACGGCATCGCGTTCGATCTGGCCCAAAAGGT
GTTCGCTGATCCGCTTCATCTCATAGTGCCTGACCGCTTTGAAGATGGCGAACAACGGTGGCATGCGATCGGGGCGATCG
GAGCCGTAGTGGTCTTGCTGGTTGTCCACACCTATCCTGATCGCGAAGATGAAGAACGGATTAGGATTGTTGGCGCCCGG
AAAGCGACACCGCATGAAAGGAGGCGCTATGAGCAAGAAGAACCTTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11095.48 Da
• Isoelectric Point: 8.6421

>AT25393 WP_013892502.1 NC_015675:1334468-1334755 [Mesorhizobium opportunistum WSM2075]
MSKKNLSRDQRDQLAKLADLADSEIDTSDIPEVPAENWVHARRGHLYRPLKQPVTIRLDADVLSWFKEHVGSGGYQTEIN
RVLRHHVIEQERRRT

Nucleotide

• Length: 288 bp

>AT25393 NC_015675:1334468-1334755 [Mesorhizobium opportunistum WSM2075]
ATGAGCAAGAAGAACCTTAGTCGGGATCAACGCGATCAACTCGCCAAGCTGGCTGACTTGGCTGACAGCGAGATTGATAC
TTCCGATATCCCGGAGGTTCCGGCGGAGAATTGGGTTCATGCTCGCCGCGGACATCTTTACCGACCACTCAAACAGCCGG
TGACCATCAGACTCGATGCTGACGTGCTATCCTGGTTCAAGGAACATGTCGGGAGCGGCGGTTATCAAACCGAAATCAAT
CGCGTGCTCCGCCATCATGTGATCGAACAGGAACGGCGTAGAACCTAA

Structures

Antitoxin

• AlphaFold DB: F7YFM1