Detailed information of TA system TA025391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 395101..395653
• Replicon: chromosome
• Accession: NC_015675
• Organism: Mesorhizobium opportunistum WSM2075

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: MESOP_RS33215
• Protein ID: WP_013891603.1
• Coordinates: 395399..395653 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: X6CU34
• Locus tag: MESOP_RS01775
• Protein ID: WP_013891602.1
• Coordinates: 395101..395412 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MESOP_RS01750	391059..391433	-	375	WP_013891597.1	hydroxyisourate hydrolase	-
MESOP_RS01755	391635..393053	+	1419	WP_013891598.1	allantoinase PuuE	-
MESOP_RS01760	393064..393927	+	864	WP_013891599.1	(S)-ureidoglycine aminohydrolase	-
MESOP_RS01765	393939..394430	+	492	WP_041164476.1	ureidoglycolate lyase	-
MESOP_RS01770	394497..395090	+	594	WP_013891601.1	nucleoside deaminase	-
MESOP_RS01775	395101..395412	-	312	WP_013891602.1	BrnA antitoxin family protein	Antitoxin
MESOP_RS33215	395399..395653	-	255	WP_013891603.1	BrnT family toxin	Toxin
MESOP_RS01780	395833..397818	+	1986	WP_013891604.1	CocE/NonD family hydrolase	-
MESOP_RS01785	397973..399559	+	1587	WP_013891605.1	twin-arginine translocation signal domain-containing protein	-
MESOP_RS01790	399576..400535	+	960	WP_013891606.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9450.12 Da
• Isoelectric Point: 10.6117

>T25391 WP_013891603.1 NC_015675:c395653-395399 [Mesorhizobium opportunistum WSM2075]
MKIVWDEPKRVTNLKNRGLDFAKLDIEFFATSTVLPAKAGRLKAIGEFGEIILAVIFKPLGSEAISVISMRRASRKERSV
YEQP

Nucleotide

• Length: 255 bp

>T25391 NC_015675:c395653-395399 [Mesorhizobium opportunistum WSM2075]
CTGAAAATCGTTTGGGATGAGCCGAAGCGCGTAACGAACCTCAAAAACCGGGGTCTCGATTTTGCCAAACTCGATATCGA
GTTTTTTGCGACTTCAACCGTCCTGCCGGCCAAGGCCGGCCGGCTCAAGGCGATTGGCGAATTCGGAGAGATCATTCTCG
CCGTTATTTTTAAGCCGCTTGGTTCCGAAGCGATTTCGGTGATCTCGATGCGTCGTGCTAGCCGAAAGGAAAGGTCAGTA
TATGAACAACCCTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11671.22 Da
• Isoelectric Point: 9.8374

>AT25391 WP_013891602.1 NC_015675:c395412-395101 [Mesorhizobium opportunistum WSM2075]
MNNPKSRIRPLTDEEEAEIQRQIAADSDDAEATDEQLARAKPFAEAFPELFESIRRARGRPPVEKPKQVVSIRLDQDVVR
KFKATGKGWQAKINEVLKNAKVS

Nucleotide

• Length: 312 bp

>AT25391 NC_015675:c395412-395101 [Mesorhizobium opportunistum WSM2075]
ATGAACAACCCTAAGTCACGTATTCGGCCGCTTACCGATGAGGAGGAAGCGGAAATCCAAAGGCAAATCGCTGCTGATTC
CGACGACGCGGAGGCGACTGACGAGCAATTGGCGCGAGCCAAGCCTTTCGCGGAAGCGTTTCCGGAACTTTTCGAAAGCA
TAAGGCGCGCTCGTGGCCGGCCTCCTGTGGAAAAGCCCAAGCAAGTTGTGTCCATCCGTCTGGATCAGGACGTCGTCAGA
AAATTCAAGGCGACTGGCAAGGGCTGGCAGGCCAAGATAAATGAGGTATTGAAAAACGCGAAGGTAAGCTAG

Structures

Antitoxin

• AlphaFold DB: X6CU34