Detailed information of TA system TA025390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 371847..372649
• Replicon: chromosome
• Accession: NC_015675
• Organism: Mesorhizobium opportunistum WSM2075

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: F7Y0J5
• Locus tag: MESOP_RS01670
• Protein ID: WP_013891581.1
• Coordinates: 372122..372649 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: F7Y0J4
• Locus tag: MESOP_RS01665
• Protein ID: WP_013891580.1
• Coordinates: 371847..372125 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MESOP_RS01640	367414..368298	-	885	WP_041163965.1	DMT family transporter	-
MESOP_RS01645	368497..368904	-	408	WP_013891576.1	DUF2000 family protein	-
MESOP_RS01650	368952..369827	+	876	WP_013891577.1	AraC family transcriptional regulator	-
MESOP_RS01655	369844..370314	-	471	WP_013891578.1	MarR family winged helix-turn-helix transcriptional regulator	-
MESOP_RS01660	370735..371787	+	1053	WP_013891579.1	GTP-binding protein	-
MESOP_RS01665	371847..372125	+	279	WP_013891580.1	DUF1778 domain-containing protein	Antitoxin
MESOP_RS01670	372122..372649	+	528	WP_013891581.1	acetyltransferase	Toxin
MESOP_RS01675	372757..373731	+	975	WP_013891582.1	WD40 repeat domain-containing protein	-
MESOP_RS01680	373747..374199	-	453	WP_013891583.1	hypothetical protein	-
MESOP_RS01685	374358..375737	+	1380	WP_013891584.1	aspartate aminotransferase family protein	-
MESOP_RS01690	375749..377149	+	1401	WP_013891585.1	glutamine synthetase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (12-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18811.55 Da
• Isoelectric Point: 9.2069

>T25390 WP_013891581.1 NC_015675:372122-372649 [Mesorhizobium opportunistum WSM2075]
VTLPSWHEEAIAKRHNRTSFDCGDAQMNDFMRRFARQSHEQNAAKTFCAIDDAAPERILGFYTIAPSAVVHGNVPAAMTK
GLARHEVSGFKLARLATDRTAAGQGLGGQLLAAAALRCLRLASEGGGILLIIDAKSERAAKWYASYGAERLQGSDLTLAM
PLATFATDLRLKGLL

Nucleotide

• Length: 528 bp

>T25390 NC_015675:372122-372649 [Mesorhizobium opportunistum WSM2075]
GTGACGCTTCCGTCCTGGCACGAAGAGGCGATTGCGAAACGGCACAATCGAACTTCCTTCGATTGCGGCGATGCGCAAAT
GAATGATTTTATGCGGCGTTTCGCACGGCAAAGTCACGAGCAGAATGCCGCCAAGACCTTCTGTGCGATTGATGACGCGG
CGCCGGAACGAATTCTCGGCTTCTACACAATCGCGCCCTCTGCCGTCGTTCATGGAAACGTGCCGGCAGCCATGACGAAG
GGCTTGGCACGGCACGAGGTTTCCGGCTTCAAGCTCGCCCGCCTCGCAACCGACCGCACCGCCGCAGGACAAGGACTTGG
AGGCCAATTGCTCGCCGCGGCGGCATTGCGCTGCCTGCGGCTTGCCAGCGAGGGTGGGGGCATCCTGCTCATTATCGACG
CCAAGAGCGAGCGCGCTGCGAAATGGTACGCCAGCTACGGTGCCGAGCGCCTTCAAGGATCGGATTTGACCCTGGCGATG
CCCCTGGCGACATTCGCAACCGATCTACGACTTAAGGGGTTGCTTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10111.70 Da
• Isoelectric Point: 8.6585

>AT25390 WP_013891580.1 NC_015675:371847-372125 [Mesorhizobium opportunistum WSM2075]
MPRTADNKTERYQIRIAPAQKAMIARAAALSHQDVADFILNKVVPEAQAVIEAAEVTKVSDRDFTRILDLLEHPPKPNAK
LRAAIAALPDTL

Nucleotide

• Length: 279 bp

>AT25390 NC_015675:371847-372125 [Mesorhizobium opportunistum WSM2075]
ATGCCGCGGACCGCCGACAATAAGACCGAGCGTTATCAAATCCGCATTGCGCCGGCGCAAAAGGCGATGATCGCGCGGGC
GGCTGCCCTGTCGCATCAGGACGTGGCTGACTTCATCCTCAACAAGGTGGTGCCCGAAGCTCAGGCCGTGATCGAAGCGG
CCGAGGTCACGAAAGTATCCGACCGCGATTTCACGCGCATACTCGACCTTTTGGAGCACCCGCCGAAACCCAACGCCAAG
CTGCGGGCAGCGATTGCGGCCTTGCCAGATACGCTGTGA

Structures

Toxin

• AlphaFold DB: F7Y0J5

Antitoxin

• AlphaFold DB: F7Y0J4