Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 660700..661329 Replicon chromosome
Accession NZ_CP102626
Organism Listeria innocua strain 2012L-5520

Toxin (Protein)


Gene name pemK Uniprot ID Q92DC7
Locus tag MXK52_RS03290 Protein ID WP_010990666.1
Coordinates 660982..661329 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7U7S1X0
Locus tag MXK52_RS03285 Protein ID WP_003766128.1
Coordinates 660700..660978 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MXK52_RS03265 (MXK52_03265) 655997..657481 + 1485 WP_003766120.1 PH domain-containing protein -
MXK52_RS03270 (MXK52_03270) 657632..659011 + 1380 WP_003766122.1 protoporphyrinogen oxidase -
MXK52_RS03275 (MXK52_03275) 659013..659369 + 357 WP_003766124.1 holo-ACP synthase -
MXK52_RS03280 (MXK52_03280) 659388..660494 + 1107 WP_003766126.1 alanine racemase -
MXK52_RS03285 (MXK52_03285) 660700..660978 + 279 WP_003766128.1 CopG family ribbon-helix-helix protein Antitoxin
MXK52_RS03290 (MXK52_03290) 660982..661329 + 348 WP_010990666.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MXK52_RS03295 (MXK52_03295) 661579..662415 + 837 WP_003766130.1 RsbT co-antagonist protein RsbRA -
MXK52_RS03300 (MXK52_03300) 662421..662777 + 357 WP_003721457.1 STAS domain-containing protein -
MXK52_RS03305 (MXK52_03305) 662780..663190 + 411 WP_031649219.1 anti-sigma regulatory factor -
MXK52_RS03310 (MXK52_03310) 663207..664211 + 1005 WP_003761312.1 PP2C family protein-serine/threonine phosphatase -
MXK52_RS03315 (MXK52_03315) 664361..664705 + 345 WP_003721460.1 anti sigma b factor antagonist RsbV -
MXK52_RS03320 (MXK52_03320) 664689..665162 + 474 WP_003761314.1 anti-sigma B factor RsbW -
MXK52_RS03325 (MXK52_03325) 665140..665919 + 780 WP_003761316.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12801.85 Da        Isoelectric Point: 6.4735

>T253894 WP_010990666.1 NZ_CP102626:660982-661329 [Listeria innocua]
MMVKRGDVYYADLSPVVGSEQGGIRPVLIIQNDIGNRFSPTVIVAAITAKIQKAKLPTHVEATRKDGFERDSVILLEQIR
TIDKQRLTDKITHLDEELMAKVNQALEVSLGVVEF

Download         Length: 348 bp

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10575.05 Da        Isoelectric Point: 4.2980

>AT253894 WP_003766128.1 NZ_CP102626:660700-660978 [Listeria innocua]
VLEKEKRTIISVELTQEMVQELDVVVEKEKMGRSEVIMEATQQFLQEKRARELRDEMERGYAEMATINFAIACECTHVEA
EAEDRNISILGG

Download         Length: 279 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E7E4N0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U7S1X0

References