Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1947110..1947747 Replicon chromosome
Accession NZ_CP102603
Organism Bacillus amyloliquefaciens strain MBLB0692

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NUW85_RS09195 Protein ID WP_003156187.1
Coordinates 1947110..1947460 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag NUW85_RS09200 Protein ID WP_003156188.1
Coordinates 1947466..1947747 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUW85_RS09155 (NUW85_09155) 1942150..1942752 - 603 WP_003156170.1 PP2C family serine/threonine-protein phosphatase -
NUW85_RS09160 (NUW85_09160) 1942752..1943540 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
NUW85_RS09165 (NUW85_09165) 1943506..1943988 - 483 WP_003156173.1 anti-sigma B factor RsbW -
NUW85_RS09170 (NUW85_09170) 1943985..1944314 - 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
NUW85_RS09175 (NUW85_09175) 1944378..1945385 - 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
NUW85_RS09180 (NUW85_09180) 1945397..1945798 - 402 WP_003156178.1 anti-sigma regulatory factor -
NUW85_RS09185 (NUW85_09185) 1945801..1946166 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
NUW85_RS09190 (NUW85_09190) 1946171..1946992 - 822 WP_003156182.1 STAS domain-containing protein -
NUW85_RS09195 (NUW85_09195) 1947110..1947460 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NUW85_RS09200 (NUW85_09200) 1947466..1947747 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NUW85_RS09205 (NUW85_09205) 1947867..1949036 - 1170 WP_003156189.1 alanine racemase -
NUW85_RS09210 (NUW85_09210) 1949153..1950160 - 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
NUW85_RS09215 (NUW85_09215) 1950325..1950690 - 366 WP_014304402.1 holo-ACP synthase -
NUW85_RS09220 (NUW85_09220) 1950783..1951382 + 600 WP_139885531.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T253885 WP_003156187.1 NZ_CP102603:c1947460-1947110 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT253885 WP_003156188.1 NZ_CP102603:c1947747-1947466 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References