Detailed information of TA system TA025388

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1706614..1707243
• Replicon: chromosome
• Accession: NC_015672
• Organism: Flexistipes sinusarabici DSM 4947

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F8E939
• Locus tag: FLEXSI_RS08075
• Protein ID: WP_013886721.1
• Coordinates: 1706842..1707243 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F8E938
• Locus tag: FLEXSI_RS08070
• Protein ID: WP_013886720.1
• Coordinates: 1706614..1706841 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FLEXSI_RS08065	1703240..1706488	+	3249	WP_013886719.1	UvrD-helicase domain-containing protein	-
FLEXSI_RS08070	1706614..1706841	+	228	WP_013886720.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FLEXSI_RS08075	1706842..1707243	+	402	WP_013886721.1	type II toxin-antitoxin system VapC family toxin	Toxin
FLEXSI_RS08080	1707265..1707711	+	447	WP_013886722.1	hypothetical protein	-
FLEXSI_RS08085	1707787..1709154	-	1368	WP_013885895.1	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (9-53)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15271.80 Da
• Isoelectric Point: 9.3113

>T25388 WP_013886721.1 NC_015672:1706842-1707243 [Flexistipes sinusarabici DSM 4947]
MSYLFDTNICIYIIKNKYKQIAKKMQEAGLEKICISAITVAELEYGISKSSKKDENRIALLEFLIPFTVLNFDQNDARKY
GQIRYNLQKAGTPIGNMDLLIGSQALARDMTLITNNEKEFSRIQGLKIENWIK

Nucleotide

• Length: 402 bp

>T25388 NC_015672:1706842-1707243 [Flexistipes sinusarabici DSM 4947]
ATGTCTTACCTTTTTGATACAAACATATGTATTTACATCATAAAAAATAAGTACAAACAAATTGCAAAAAAAATGCAGGA
AGCTGGATTAGAAAAAATTTGTATCTCTGCTATTACCGTTGCAGAGTTAGAATACGGTATATCAAAAAGCTCCAAAAAGG
ACGAAAACAGGATTGCGTTGCTGGAATTCCTTATTCCGTTTACAGTATTAAATTTTGATCAAAATGATGCCAGAAAATAC
GGTCAAATCAGGTACAATTTGCAAAAAGCAGGCACACCTATTGGTAACATGGATTTGCTTATTGGATCCCAGGCTTTGGC
AAGAGATATGACTCTGATAACAAATAACGAAAAAGAATTCAGCCGGATACAAGGGTTAAAAATAGAAAACTGGATTAAAT
AG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8825.00 Da
• Isoelectric Point: 4.6210

>AT25388 WP_013886720.1 NC_015672:1706614-1706841 [Flexistipes sinusarabici DSM 4947]
MDTAKIFENGKSQAVRLPKKYRFDSDDVFIKKIDDIVVLIPKDKVWQIFSSSLDKFSEDVDFQKDNSIPQEREEI

Nucleotide

• Length: 228 bp

>AT25388 NC_015672:1706614-1706841 [Flexistipes sinusarabici DSM 4947]
ATGGATACTGCAAAAATATTTGAAAACGGAAAAAGTCAGGCTGTCAGGTTACCCAAAAAATACAGGTTTGACAGCGATGA
TGTGTTTATAAAAAAAATTGATGACATCGTTGTATTAATTCCTAAAGATAAAGTCTGGCAAATTTTTAGTTCATCTTTGG
ACAAATTTTCGGAAGATGTAGATTTTCAAAAAGACAATTCTATACCACAGGAAAGAGAAGAAATATAA

Structures

Toxin

• AlphaFold DB: F8E939

Antitoxin

• AlphaFold DB: F8E938