Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5201286..5201911 Replicon chromosome
Accession NZ_CP102552
Organism Klebsiella pneumoniae strain KPH3

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NUG48_RS25660 Protein ID WP_002882817.1
Coordinates 5201286..5201669 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NUG48_RS25665 Protein ID WP_004150355.1
Coordinates 5201669..5201911 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUG48_RS25645 (NUG48_25650) 5198652..5199554 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NUG48_RS25650 (NUG48_25655) 5199551..5200186 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NUG48_RS25655 (NUG48_25660) 5200183..5201112 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NUG48_RS25660 (NUG48_25665) 5201286..5201669 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NUG48_RS25665 (NUG48_25670) 5201669..5201911 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NUG48_RS25670 (NUG48_25675) 5202116..5203033 + 918 WP_002882812.1 alpha/beta hydrolase -
NUG48_RS25675 (NUG48_25680) 5203047..5203988 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NUG48_RS25680 (NUG48_25685) 5204033..5204470 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NUG48_RS25685 (NUG48_25690) 5204467..5205327 - 861 WP_002882807.1 virulence factor BrkB family protein -
NUG48_RS25690 (NUG48_25695) 5205321..5205920 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T253863 WP_002882817.1 NZ_CP102552:c5201669-5201286 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT253863 WP_004150355.1 NZ_CP102552:c5201911-5201669 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References