Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4615290..4616100 Replicon chromosome
Accession NZ_CP102552
Organism Klebsiella pneumoniae strain KPH3

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag NUG48_RS22855 Protein ID WP_002887280.1
Coordinates 4615290..4615823 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NUG48_RS22860 Protein ID WP_002887278.1
Coordinates 4615834..4616100 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUG48_RS22850 (NUG48_22855) 4614121..4615242 + 1122 WP_002887282.1 cupin domain-containing protein -
NUG48_RS22855 (NUG48_22860) 4615290..4615823 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
NUG48_RS22860 (NUG48_22865) 4615834..4616100 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NUG48_RS22865 (NUG48_22870) 4616203..4617636 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
NUG48_RS22870 (NUG48_22875) 4617626..4618309 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NUG48_RS22875 (NUG48_22880) 4618481..4619866 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
NUG48_RS22880 (NUG48_22885) 4619884..4620228 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T253860 WP_002887280.1 NZ_CP102552:c4615823-4615290 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT253860 WP_002887278.1 NZ_CP102552:c4616100-4615834 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References