Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 984404..984959 Replicon chromosome
Accession NZ_CP102536
Organism Bifidobacterium breve strain BIF195

Toxin (Protein)


Gene name mazF Uniprot ID B7GSS5
Locus tag NUU00_RS04155 Protein ID WP_003833460.1
Coordinates 984404..984727 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag NUU00_RS04160 Protein ID WP_003829112.1
Coordinates 984714..984959 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUU00_RS04130 (NUU00_04130) 979441..979729 - 289 Protein_799 IS256 family transposase -
NUU00_RS04135 (NUU00_04135) 980200..981465 - 1266 WP_025262925.1 Fic family protein -
NUU00_RS04140 (NUU00_04140) 982007..982372 - 366 WP_013140585.1 YccF domain-containing protein -
NUU00_RS04145 (NUU00_04145) 982610..983479 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
NUU00_RS04150 (NUU00_04150) 983573..984187 + 615 WP_003829107.1 cupin domain-containing protein -
NUU00_RS04155 (NUU00_04155) 984404..984727 - 324 WP_003833460.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NUU00_RS04160 (NUU00_04160) 984714..984959 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
NUU00_RS04165 (NUU00_04165) 985133..986389 + 1257 WP_003829116.1 HipA domain-containing protein -
NUU00_RS04170 (NUU00_04170) 986654..986845 + 192 WP_003829118.1 hypothetical protein -
NUU00_RS04175 (NUU00_04175) 987029..988300 - 1272 WP_003829121.1 MFS transporter -
NUU00_RS04180 (NUU00_04180) 988457..989119 - 663 WP_025262926.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 974174..1022459 48285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11829.64 Da        Isoelectric Point: 6.2932

>T253816 WP_003833460.1 NZ_CP102536:c984727-984404 [Bifidobacterium breve]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT253816 WP_003829112.1 NZ_CP102536:c984959-984714 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0SL31

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References