Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 359630..360201 Replicon chromosome
Accession NZ_CP102534
Organism Enterococcus faecium strain ENCfa-68

Toxin (Protein)


Gene name mazF Uniprot ID R2Q2D5
Locus tag NUU03_RS01745 Protein ID WP_002341043.1
Coordinates 359860..360201 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2A4E1M8
Locus tag NUU03_RS01740 Protein ID WP_025477367.1
Coordinates 359630..359860 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUU03_RS01720 (NUU03_01705) 355894..356487 + 594 WP_002342793.1 malonate decarboxylase holo-ACP synthase -
NUU03_RS01725 (NUU03_01710) 356511..357596 + 1086 WP_002342794.1 acyltransferase family protein -
NUU03_RS01730 (NUU03_01715) 357618..358487 + 870 WP_002342795.1 triphosphoribosyl-dephospho-CoA synthase MdcB -
NUU03_RS01735 (NUU03_01720) 358599..359363 + 765 WP_002341045.1 TIM44-like domain-containing protein -
NUU03_RS01740 (NUU03_01725) 359630..359860 + 231 WP_025477367.1 hypothetical protein Antitoxin
NUU03_RS01745 (NUU03_01730) 359860..360201 + 342 WP_002341043.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NUU03_RS01750 360381..360740 + 360 WP_156238084.1 hypothetical protein -
NUU03_RS01755 (NUU03_01735) 360917..362998 + 2082 WP_002342796.1 PTS sugar transporter subunit IIA -
NUU03_RS01760 (NUU03_01740) 363034..363336 + 303 Protein_351 PTS sugar transporter subunit IIB -
NUU03_RS01765 (NUU03_01745) 363362..364651 + 1290 WP_002341041.1 PTS sugar transporter subunit IIC -
NUU03_RS01770 (NUU03_01750) 364670..365077 + 408 Protein_353 tetratricopeptide repeat protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13316.66 Da        Isoelectric Point: 9.9258

>T253814 WP_002341043.1 NZ_CP102534:359860-360201 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQ
LKSLDFKERRLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8566.42 Da        Isoelectric Point: 4.0070

>AT253814 WP_025477367.1 NZ_CP102534:359630-359860 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829AD04

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4E1M8

References