253799

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 46149..46952 Replicon plasmid pCF10-NDM
Accession NZ_CP102502
Organism Citrobacter youngae strain CF10

Toxin (Protein)


Gene name KacT Uniprot ID W8E895
Locus tag NUG39_RS25325 Protein ID WP_016479963.1
Coordinates 46422..46952 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag NUG39_RS25320 Protein ID WP_022652312.1
Coordinates 46149..46418 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUG39_RS25305 (NUG39_25305) 42635..42751 - 117 Protein_43 IS5 family transposase -
NUG39_RS25310 (NUG39_25310) 43269..44008 + 740 Protein_44 site-specific integrase -
NUG39_RS25315 (NUG39_25315) 44304..45293 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
NUG39_RS25320 (NUG39_25320) 46149..46418 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
NUG39_RS25325 (NUG39_25325) 46422..46952 + 531 WP_016479963.1 GNAT family N-acetyltransferase Toxin
NUG39_RS25330 (NUG39_25330) 47120..48100 + 981 WP_000019403.1 IS5-like element IS5 family transposase -
NUG39_RS25335 (NUG39_25335) 48247..48993 - 747 Protein_49 IS3 family transposase -
NUG39_RS25340 (NUG39_25340) 49006..49125 - 120 Protein_50 IS30 family transposase -
NUG39_RS25345 (NUG39_25345) 49380..49562 - 183 WP_016479964.1 hypothetical protein -
NUG39_RS25350 (NUG39_25350) 49751..51391 - 1641 WP_004201176.1 chaperonin GroEL -
NUG39_RS25355 (NUG39_25355) 51447..51737 - 291 WP_004201172.1 co-chaperone GroES -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 htpB 1..138196 138196
- inside IScluster/Tn - - 40668..48912 8244


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20350.24 Da        Isoelectric Point: 6.4907

>T253799 WP_016479963.1 NZ_CP102502:46422-46952 [Citrobacter youngae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT253799 WP_022652312.1 NZ_CP102502:46149-46418 [Citrobacter youngae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0L7Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References