253794

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 89918..91541 Replicon plasmid pCF10-tmexCD1
Accession NZ_CP102501
Organism Citrobacter youngae strain CF10

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag NUG39_RS24490 Protein ID WP_000088044.1
Coordinates 90216..91541 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag NUG39_RS24485 Protein ID WP_012477377.1
Coordinates 89918..90211 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUG39_RS24465 (NUG39_24465) 85717..86109 + 393 WP_022652193.1 hypothetical protein -
NUG39_RS24470 (NUG39_24470) 86768..87424 + 657 WP_000462754.1 trypsin-like serine protease -
NUG39_RS24475 (NUG39_24475) 87627..88124 + 498 WP_000340139.1 membrane protein -
NUG39_RS24480 (NUG39_24480) 88129..89517 + 1389 WP_000083579.1 hypothetical protein -
NUG39_RS24485 (NUG39_24485) 89918..90211 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
NUG39_RS24490 (NUG39_24490) 90216..91541 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
NUG39_RS24495 (NUG39_24495) 91602..91808 + 207 WP_000134171.1 hypothetical protein -
NUG39_RS24500 (NUG39_24500) 91909..92319 + 411 WP_000985909.1 hypothetical protein -
NUG39_RS24505 (NUG39_24505) 92332..93147 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
NUG39_RS24510 (NUG39_24510) 93400..93825 + 426 WP_000278471.1 hypothetical protein -
NUG39_RS24515 (NUG39_24515) 94374..94682 + 309 WP_001224686.1 hypothetical protein -
NUG39_RS24520 (NUG39_24520) 94698..95555 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / mph(A) / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / qnrS1 - 1..212154 212154


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T253794 WP_000088044.1 NZ_CP102501:90216-91541 [Citrobacter youngae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT253794 WP_012477377.1 NZ_CP102501:89918-90211 [Citrobacter youngae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References