Detailed information of TA system TA253780

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1270198..1270876
• Replicon: chromosome
• Accession: NZ_CP102500
• Organism: Citrobacter youngae strain CF10

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: NUG39_RS06190
• Protein ID: WP_257748941.1
• Coordinates: 1270198..1270521 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: NUG39_RS06195
• Protein ID: WP_257748943.1
• Coordinates: 1270553..1270876 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NUG39_RS06175 (1266760)	1266760..1267218	-	459	WP_048212960.1	GNAT family N-acetyltransferase	-
NUG39_RS06180 (1267345)	1267345..1268814	+	1470	WP_257749312.1	PLP-dependent aminotransferase family protein	-
NUG39_RS06185 (1269043)	1269043..1269768	+	726	WP_257748939.1	GNAT family protein	-
NUG39_RS06190 (1270198)	1270198..1270521	-	324	WP_257748941.1	toxin	Toxin
NUG39_RS06195 (1270553)	1270553..1270876	-	324	WP_257748943.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
NUG39_RS06200 (1270898)	1270898..1271119	-	222	WP_257748945.1	DUF987 domain-containing protein	-
NUG39_RS06205 (1271128)	1271128..1271598	-	471	WP_032642914.1	DNA repair protein RadC	-
NUG39_RS06210 (1271631)	1271631..1272449	-	819	WP_257748946.1	DUF932 domain-containing protein	-
NUG39_RS06215 (1272708)	1272708..1273070	-	363	WP_257748947.1	hypothetical protein	-
NUG39_RS06220 (1273102)	1273102..1273542	-	441	WP_257748948.1	hypothetical protein	-
NUG39_RS06225 (1273551)	1273551..1274237	-	687	WP_257748949.1	WYL domain-containing protein	-
NUG39_RS06230 (1274258)	1274258..1274995	-	738	WP_257748950.1	DUF3944 domain-containing protein	-
NUG39_RS06235 (1275137)	1275137..1275715	-	579	WP_257748951.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (4-102)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12042.88 Da
• Isoelectric Point: 6.4747

>T253780 WP_257748941.1 NZ_CP102500:c1270521-1270198 [Citrobacter youngae]
MQISTVPATVPVSSRLPPVQVWQQLLTYLLEHHYGLALNDTPFHNDTAIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQTPFITATDILRAKKSIGLLPCS

Nucleotide

• Length: 324 bp

>T253780 NZ_CP102500:c1270521-1270198 [Citrobacter youngae]
ATGCAAATTTCAACTGTACCAGCCACGGTGCCGGTTTCGTCACGCCTGCCGCCCGTACAGGTCTGGCAGCAATTGTTAAC
GTATCTACTGGAACACCATTACGGCCTGGCTCTCAACGACACGCCATTCCACAACGATACGGCCATTAAGGAGCATATCG
ACGCGGGAATCACGCTTGCTGATGCTGTTAATTTCCTGGTCGAAAAATACGAACTAGTTCGTATCGACCGGAAGGGATTT
TCATGGCAAGAGCAAACGCCCTTCATTACCGCAACGGATATTCTGCGTGCCAAAAAGAGCATAGGGTTGTTGCCCTGCTC
TTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11994.67 Da
• Isoelectric Point: 6.4764

>AT253780 WP_257748943.1 NZ_CP102500:c1270876-1270553 [Citrobacter youngae]
MKNSWGLKRDISPCFGARLVQEGCRLHFLADRAGISGAFNEEEALRLDQAFPLVMKQLELMLTTDELNPRHQHCVTLHHN
GLTCEADTLGSHGYVYIAIYPQTPASQ

Nucleotide

• Length: 324 bp

>AT253780 NZ_CP102500:c1270876-1270553 [Citrobacter youngae]
ATGAAAAATTCATGGGGACTGAAGCGCGACATCTCGCCGTGCTTCGGTGCAAGGCTTGTGCAGGAAGGCTGTCGCCTGCA
TTTTCTGGCAGACCGTGCAGGTATTAGCGGTGCGTTTAACGAGGAGGAGGCGCTGCGTCTGGATCAGGCATTTCCGCTAG
TGATGAAACAGCTTGAATTGATGCTGACAACCGATGAACTGAATCCGCGCCATCAGCATTGCGTCACGTTGCATCATAAC
GGACTCACCTGCGAGGCGGATACTCTCGGTTCTCATGGCTATGTTTACATCGCTATTTATCCTCAGACACCTGCTAGCCA
ATAA