Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2767998..2768617 Replicon chromosome
Accession NC_015663
Organism Enterobacter aerogenes KCTC 2190

Toxin (Protein)


Gene name Hha Uniprot ID A0A0H3FXE2
Locus tag EAE_RS13155 Protein ID WP_015367918.1
Coordinates 2767998..2768216 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H3FPM3
Locus tag EAE_RS13160 Protein ID WP_015367917.1
Coordinates 2768243..2768617 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAE_RS13125 2764071..2764331 + 261 WP_015367923.1 type B 50S ribosomal protein L31 -
EAE_RS13130 2764334..2764474 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
EAE_RS13135 2764471..2765181 - 711 WP_015704607.1 GNAT family N-acetyltransferase -
EAE_RS13140 2765284..2766738 + 1455 WP_015704608.1 PLP-dependent aminotransferase family protein -
EAE_RS13145 2766713..2767177 - 465 WP_015367920.1 YlaC family protein -
EAE_RS13150 2767303..2767866 - 564 WP_015704609.1 maltose O-acetyltransferase -
EAE_RS13155 2767998..2768216 - 219 WP_015367918.1 hemolysin expression modulator Hha Toxin
EAE_RS13160 2768243..2768617 - 375 WP_015367917.1 Hha toxicity modulator TomB Antitoxin
EAE_RS13165 2769089..2772235 - 3147 WP_015704610.1 multidrug efflux RND transporter permease subunit -
EAE_RS13170 2772258..2773457 - 1200 WP_020079451.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2759332..2768617 9285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8569.96 Da        Isoelectric Point: 9.4828

>T25377 WP_015367918.1 NC_015663:c2768216-2767998 [Klebsiella aerogenes KCTC 2190]
MSGKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T25377 NC_015663:c2768216-2767998 [Klebsiella aerogenes KCTC 2190]
ATGTCTGGTAAACCATTAACTAAAACAGACTATTTGATGCGTTTACGCCGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAGAATAAATATGAACTTTCCGACAATGAATTGGCGGTATTTTACTCAGCAGCCGACCATCGTCTTGCTGAAT
TGACCATGAATAAGCTATACGACAAAATCCCAACTTCAGTATGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14464.21 Da        Isoelectric Point: 4.9045

>AT25377 WP_015367917.1 NC_015663:c2768617-2768243 [Klebsiella aerogenes KCTC 2190]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNRGWVNDPTSAVNLQLNELIEHIATYALNYKIKYAEDNKLISQLDEYL
DDTFMLFSSYGINTSDLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT25377 NC_015663:c2768617-2768243 [Klebsiella aerogenes KCTC 2190]
ATGGACGAGTACTCGCCAAAAAGACATGATATCGCGCAGCTAAGATTTCTCTGCGAAACGTTGTATCATGACTGCCTTGC
AAACCTGGAAGAAAGCAACCGCGGCTGGGTTAACGATCCCACCTCGGCGGTGAATCTTCAATTAAATGAATTGATCGAGC
ACATAGCAACTTACGCGCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATTTCTCAGCTTGACGAATACCTG
GATGACACTTTTATGCTGTTCAGTAGCTATGGCATAAACACAAGCGATCTCCAAAAATGGCGCAAATCCGGCAATCGGTT
ATTCCGATGCTTTGTGAACGCCAGCCGGGAGAACCCGGCAAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FPM3

References