Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 53223..53748 Replicon plasmid p113Kb
Accession NZ_CP102489
Organism Escherichia coli O15:H18 strain ST69

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag NUI00_RS26130 Protein ID WP_001159871.1
Coordinates 53443..53748 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag NUI00_RS26125 Protein ID WP_000813639.1
Coordinates 53223..53441 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUI00_RS26120 (49541) 49541..52672 + 3132 WP_115763486.1 hypothetical protein -
NUI00_RS26125 (53223) 53223..53441 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
NUI00_RS26130 (53443) 53443..53748 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
NUI00_RS26135 (53749) 53749..54555 + 807 WP_001774328.1 site-specific integrase -
NUI00_RS26140 (55417) 55417..56171 + 755 Protein_36 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iroN / iroE / iroD / iroC / iroB 1..113589 113589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T253760 WP_001159871.1 NZ_CP102489:53443-53748 [Escherichia coli O15:H18]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT253760 WP_000813639.1 NZ_CP102489:53223-53441 [Escherichia coli O15:H18]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References