Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3273215..3273712 Replicon chromosome
Accession NZ_CP102488
Organism Escherichia coli O15:H18 strain ST69

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NUI00_RS16025 Protein ID WP_001173343.1
Coordinates 3273215..3273388 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NUI00_RS16030 Protein ID WP_000398619.1
Coordinates 3273416..3273712 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUI00_RS16015 (3269886) 3269886..3271343 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
NUI00_RS16020 (3271580) 3271580..3273097 + 1518 WP_000003806.1 lysine--tRNA ligase -
NUI00_RS16025 (3273215) 3273215..3273388 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NUI00_RS16030 (3273416) 3273416..3273712 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NUI00_RS16035 (3273941) 3273941..3274213 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NUI00_RS16040 (3274225) 3274225..3274455 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
NUI00_RS16045 (3274636) 3274636..3276267 + 1632 WP_001216477.1 sensor histidine kinase -
NUI00_RS16050 (3276264) 3276264..3276983 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T253751 WP_001173343.1 NZ_CP102488:c3273388-3273215 [Escherichia coli O15:H18]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT253751 WP_000398619.1 NZ_CP102488:c3273712-3273416 [Escherichia coli O15:H18]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References