Detailed information of TA system TA025371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 4859240..4859868
• Replicon: chromosome
• Accession: NC_015656
• Organism: Frankia symbiont of Datisca glomerata

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F8AWY4
• Locus tag: FSYMDG_RS20475
• Protein ID: WP_013875296.1
• Coordinates: 4859485..4859868 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F8AWY3
• Locus tag: FSYMDG_RS20470
• Protein ID: WP_013875295.1
• Coordinates: 4859240..4859488 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSYMDG_RS20450	4855427..4856689	+	1263	WP_013872077.1	IS4 family transposase	-
FSYMDG_RS20455	4856955..4858153	-	1199	Protein_4031	IS3 family transposase	-
FSYMDG_RS20465	4858254..4858583	+	330	WP_013875293.1	FKBP-type peptidyl-prolyl cis-trans isomerase	-
FSYMDG_RS26925	4858946..4859083	-	138	WP_013875294.1	hypothetical protein	-
FSYMDG_RS20470	4859240..4859488	+	249	WP_013875295.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FSYMDG_RS20475	4859485..4859868	+	384	WP_013875296.1	type II toxin-antitoxin system VapC family toxin	Toxin
FSYMDG_RS20480	4859942..4860570	-	629	Protein_4036	Fe-S cluster assembly protein HesB	-
FSYMDG_RS20485	4860822..4861085	+	264	WP_202797747.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
FSYMDG_RS20490	4861085..4861501	+	417	WP_013875298.1	type II toxin-antitoxin system VapC family toxin	-
FSYMDG_RS26930	4861498..4861638	-	141	WP_165001457.1	hypothetical protein	-
FSYMDG_RS20500	4862395..4862592	+	198	WP_131772890.1	hypothetical protein	-
FSYMDG_RS20505	4862589..4864283	+	1695	WP_013875300.1	N-6 DNA methylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (21-111)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-63)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13581.65 Da
• Isoelectric Point: 6.4697

>T25371 WP_013875296.1 NC_015656:4859485-4859868 [Candidatus Frankia datiscae]
MSRGILDTSVLVATDVAPIPGELAVSVISIAELQFGVLVARTPEARANRLARLSALQRRFDPLPVDDAVADSYGRLAARV
VEIGRQPRARTMDLLIAATAHAHEASVYTRNAADLAGLEDLVRIVTI

Nucleotide

• Length: 384 bp

>T25371 NC_015656:4859485-4859868 [Candidatus Frankia datiscae]
ATGAGCCGCGGCATTCTGGACACCAGCGTGCTGGTCGCCACCGACGTCGCCCCGATCCCCGGTGAACTGGCGGTCAGCGT
CATCAGCATCGCCGAGCTCCAGTTCGGCGTCCTCGTCGCCAGAACACCCGAGGCCCGCGCGAACCGACTCGCCCGTCTCA
GCGCACTCCAACGACGATTCGACCCGTTACCCGTCGACGATGCTGTCGCCGACAGTTACGGACGACTCGCTGCCCGGGTC
GTCGAAATCGGACGCCAGCCACGCGCACGAACGATGGACCTGCTGATCGCGGCCACCGCGCACGCCCACGAAGCCAGCGT
CTATACCCGCAACGCCGCCGACCTCGCCGGCCTGGAAGACCTCGTCAGAATCGTGACGATCTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9186.25 Da
• Isoelectric Point: 4.5600

>AT25371 WP_013875295.1 NC_015656:4859240-4859488 [Candidatus Frankia datiscae]
MSEIGLREVRQNASELVRRAQAGERLTITVSGRPAAVLGPVTPRAWRRWDDLADVFTLPTDADWPSDRELVDGTLTDPWG
DR

Nucleotide

• Length: 249 bp

>AT25371 NC_015656:4859240-4859488 [Candidatus Frankia datiscae]
ATGAGTGAGATAGGGCTTCGCGAGGTGCGGCAGAACGCCAGCGAGCTGGTGCGACGCGCCCAGGCCGGCGAGCGGTTGAC
CATCACTGTTTCCGGCCGGCCGGCAGCCGTTCTCGGCCCGGTCACCCCACGCGCGTGGCGGCGCTGGGACGACCTCGCGG
ACGTCTTCACCCTGCCGACCGACGCCGACTGGCCCAGCGATCGCGAGCTCGTCGACGGCACCCTCACCGATCCCTGGGGA
GATCGATGA

Structures

Toxin

• AlphaFold DB: F8AWY4

Antitoxin

• AlphaFold DB: F8AWY3