Detailed information of TA system TA025370

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 4060113..4060699
• Replicon: chromosome
• Accession: NC_015656
• Organism: Frankia symbiont of Datisca glomerata

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8B1H8
• Locus tag: FSYMDG_RS16970
• Protein ID: WP_013874619.1
• Coordinates: 4060113..4060403 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8B1H9
• Locus tag: FSYMDG_RS16975
• Protein ID: WP_013874620.1
• Coordinates: 4060400..4060699 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSYMDG_RS16935	4055240..4055440	-	201	WP_043603892.1	hypothetical protein	-
FSYMDG_RS16940	4055437..4056651	-	1215	WP_043606884.1	acyl-CoA dehydrogenase	-
FSYMDG_RS16945	4056919..4057938	+	1020	WP_083828326.1	formyltetrahydrofolate deformylase	-
FSYMDG_RS16950	4057908..4058576	-	669	WP_013874615.1	phosphatase PAP2 family protein	-
FSYMDG_RS16955	4059088..4059366	+	279	WP_131772750.1	Arc family DNA-binding protein	-
FSYMDG_RS16960	4059363..4059704	+	342	WP_013874617.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FSYMDG_RS16965	4059830..4060018	-	189	WP_013874618.1	NUDIX hydrolase	-
FSYMDG_RS16970	4060113..4060403	-	291	WP_013874619.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FSYMDG_RS16975	4060400..4060699	-	300	WP_013874620.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
FSYMDG_RS16980	4060944..4062031	-	1088	Protein_3345	IS630 family transposase	-
FSYMDG_RS16985	4062006..4063823	+	1818	WP_085948566.1	protein kinase	-
FSYMDG_RS16990	4063945..4064859	+	915	WP_131772754.1	MurR/RpiR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4060944..4061156	212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (26-93)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10830.23 Da
• Isoelectric Point: 9.5857

>T25370 WP_013874619.1 NC_015656:c4060403-4060113 [Candidatus Frankia datiscae]
VTGTGEQPFELRITGPAARALASRLPEKIAAAVHEFVTTTLLDNPHRLGRRLLLPPYEGTWSARRGSYRVLYEIDEDNRI
VTVTAVEHRSDAYRSR

Nucleotide

• Length: 291 bp

>T25370 NC_015656:c4060403-4060113 [Candidatus Frankia datiscae]
GTGACCGGAACTGGCGAGCAGCCGTTTGAACTTCGGATCACTGGTCCTGCGGCGCGTGCTCTCGCCAGCCGGTTGCCCGA
GAAGATCGCGGCTGCCGTCCACGAGTTCGTCACGACCACACTCCTGGACAATCCTCACCGGCTTGGCAGACGCCTCCTGC
TTCCACCGTACGAGGGCACCTGGTCCGCTCGTCGCGGCAGTTACCGGGTGTTGTATGAAATCGACGAGGACAACCGCATT
GTTACCGTGACCGCCGTCGAACATCGCTCCGACGCGTACCGCTCCCGCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11029.42 Da
• Isoelectric Point: 4.9737

>AT25370 WP_013874620.1 NC_015656:c4060699-4060400 [Candidatus Frankia datiscae]
MTVMPFTEARNRLSELIDEVGRTHERVEITRHGHAVAVLISPDDLAALEEALDVLASREAMRQLAESRDARESGDVLDAE
ELAALMAKRARRAESPHTR

Nucleotide

• Length: 300 bp

>AT25370 NC_015656:c4060699-4060400 [Candidatus Frankia datiscae]
ATGACTGTCATGCCGTTCACCGAGGCGCGCAACCGGCTGTCGGAGCTCATTGACGAGGTGGGGCGGACTCATGAGCGAGT
GGAGATCACCCGTCACGGGCATGCGGTGGCGGTCCTGATCTCTCCCGATGACCTGGCGGCGCTGGAGGAGGCGCTGGATG
TGTTGGCGAGTCGGGAGGCGATGCGGCAGCTTGCGGAATCCCGGGACGCCCGCGAGTCCGGCGACGTGCTCGATGCCGAG
GAGCTCGCCGCGCTCATGGCGAAACGGGCGCGCCGCGCCGAGTCGCCCCACACACGGTGA

Structures

Toxin

• AlphaFold DB: F8B1H8

Antitoxin

• AlphaFold DB: F8B1H9