Detailed information of TA system TA025369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 3458283..3459055
• Replicon: chromosome
• Accession: NC_015656
• Organism: Frankia symbiont of Datisca glomerata

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: F8B6M3
• Locus tag: FSYMDG_RS14365
• Protein ID: WP_013874133.1
• Coordinates: 3458543..3459055 (+)
• Length: 171 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: F8B6M2
• Locus tag: FSYMDG_RS14360
• Protein ID: WP_013874132.1
• Coordinates: 3458283..3458546 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSYMDG_RS14350	3454302..3456104	-	1803	WP_013874130.1	AMP-dependent synthetase/ligase	-
FSYMDG_RS14355	3456648..3458174	+	1527	WP_013874131.1	MFS transporter	-
FSYMDG_RS14360	3458283..3458546	+	264	WP_013874132.1	DUF1778 domain-containing protein	Antitoxin
FSYMDG_RS14365	3458543..3459055	+	513	WP_013874133.1	GNAT family N-acetyltransferase	Toxin
FSYMDG_RS14370	3459507..3460070	+	564	WP_083828497.1	PaaI family thioesterase	-
FSYMDG_RS14375	3460113..3460796	-	684	WP_131774012.1	HAD family phosphatase	-
FSYMDG_RS14380	3460866..3461717	-	852	WP_013874136.1	MBL fold metallo-hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-81)

Sequences

Toxin

Protein

• Length: 171 a.a.
• Molecular weight: 18372.93 Da
• Isoelectric Point: 9.0667

>T25369 WP_013874133.1 NC_015656:3458543-3459055 [Candidatus Frankia datiscae]
VSGYSAPRPIAAADDLSTFDSGESSLDDWLRRRALGNQTAGASRCFVTCRDGRVVGYYALATGAVQRLDTSRRIGQGMPD
PVPVILLGRLAVDVKEQGRGLGAHLLRDAITRTVAAAEIVGVRALLVHALHDQARQFYLYFGFEPSPTHPLHLFLLMKDA
RALTRGDTSG

Nucleotide

• Length: 513 bp

>T25369 NC_015656:3458543-3459055 [Candidatus Frankia datiscae]
GTGAGTGGTTACTCCGCACCCAGGCCGATCGCCGCGGCTGATGACCTGTCGACCTTTGACAGCGGCGAGTCCTCACTGGA
CGACTGGTTGCGCCGACGGGCGTTGGGTAACCAGACAGCGGGAGCCTCCCGCTGCTTTGTCACGTGCCGTGACGGACGTG
TCGTCGGCTACTACGCCCTGGCTACCGGAGCTGTACAGCGGCTGGACACCAGCCGACGCATCGGCCAGGGCATGCCCGAT
CCCGTCCCGGTCATTCTGCTGGGGCGTCTCGCCGTCGACGTCAAGGAACAGGGCCGGGGTCTGGGCGCCCATCTGTTACG
CGACGCGATCACCCGCACCGTCGCGGCAGCGGAGATCGTCGGTGTGCGAGCCCTGCTCGTACACGCGCTGCACGACCAGG
CTCGGCAGTTCTACCTGTATTTCGGGTTTGAACCGTCTCCCACCCATCCGCTCCATCTGTTCCTTCTGATGAAGGATGCC
CGGGCTCTGACCAGAGGCGACACATCCGGGTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.27 Da
• Isoelectric Point: 5.1745

>AT25369 WP_013874132.1 NC_015656:3458283-3458546 [Candidatus Frankia datiscae]
MIKSSRIAVRLPEEQDALIRAAAEVEGSTVTEFTVNAAVARAKDVLADQRLFTLGSEAWTEFLTILDRPVTRKPRLERLF
TEKSIFE

Nucleotide

• Length: 264 bp

>AT25369 NC_015656:3458283-3458546 [Candidatus Frankia datiscae]
ATGATCAAAAGTTCCCGCATCGCTGTGCGCCTGCCTGAAGAGCAGGATGCGCTGATCCGCGCGGCAGCCGAGGTCGAAGG
CTCGACCGTCACCGAGTTCACCGTGAATGCGGCCGTAGCCAGGGCCAAGGACGTCCTGGCAGACCAGCGCCTGTTCACAC
TGGGGAGCGAGGCATGGACGGAGTTCCTCACCATTCTGGATCGTCCGGTTACCCGCAAACCGCGCCTGGAACGCCTGTTC
ACCGAGAAGTCGATCTTTGAGTGA

Structures

Toxin

• AlphaFold DB: F8B6M3

Antitoxin

• AlphaFold DB: F8B6M2