Detailed information of TA system TA253680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 2563859..2564559
• Replicon: chromosome
• Accession: NZ_CP102458
• Organism: Burkholderia multivorans strain NML180963

Toxin (Protein)

• Gene name: tad
• Uniprot ID: B9B4U7
• Locus tag: NUJ28_RS12450
• Protein ID: WP_006398693.1
• Coordinates: 2563859..2564236 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: NUJ28_RS12455
• Protein ID: WP_054317404.1
• Coordinates: 2564236..2564559 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NUJ28_RS12425 (NUJ28_12425)	2559388..2559837	+	450	WP_006413500.1	VOC family protein	-
NUJ28_RS12430 (NUJ28_12430)	2559941..2561491	-	1551	WP_164677954.1	sodium:solute symporter	-
NUJ28_RS12435 (NUJ28_12435)	2561488..2561721	-	234	WP_006398697.1	DUF3311 domain-containing protein	-
NUJ28_RS12440 (NUJ28_12440)	2561918..2562835	-	918	WP_176034451.1	spermidine synthase	-
NUJ28_RS12445 (NUJ28_12445)	2563063..2563317	+	255	WP_105777956.1	hypothetical protein	-
NUJ28_RS12450 (NUJ28_12450)	2563859..2564236	+	378	WP_006398693.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NUJ28_RS12455 (NUJ28_12455)	2564236..2564559	+	324	WP_054317404.1	helix-turn-helix transcriptional regulator	Antitoxin
NUJ28_RS12460 (NUJ28_12460)	2564582..2565073	-	492	WP_176034450.1	DNA-deoxyinosine glycosylase	-
NUJ28_RS12465 (NUJ28_12465)	2565070..2565684	-	615	WP_176034449.1	chorismate lyase	-
NUJ28_RS12470 (NUJ28_12470)	2565693..2567591	-	1899	WP_039219510.1	molecular chaperone HtpG	-
NUJ28_RS12475 (NUJ28_12475)	2567750..2569210	-	1461	WP_105780126.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (30-85)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13504.53 Da
• Isoelectric Point: 9.7721

>T253680 WP_006398693.1 NZ_CP102458:2563859-2564236 [Burkholderia multivorans]
MGINSSMVPYTKPLYWLGSARKDLKAMPEAVQDTFGYALYLAQTGGKHDQAKPLRGFGSAGVLEVVESEDSGTYRAVYTV
KLGGAVYVLHCFQKKSCRGIATPKPDIELVRARLRAAEAHANGGK

Nucleotide

• Length: 378 bp

>T253680 NZ_CP102458:2563859-2564236 [Burkholderia multivorans]
ATGGGTATAAATTCGTCGATGGTTCCATACACTAAACCGCTCTACTGGCTCGGCTCGGCACGCAAGGACTTGAAAGCGAT
GCCCGAAGCGGTACAGGACACGTTCGGCTATGCGCTGTATCTGGCCCAGACGGGAGGAAAGCACGATCAGGCCAAGCCGT
TGAGGGGTTTCGGATCGGCCGGCGTGCTCGAGGTCGTGGAGTCGGAGGACAGCGGCACGTACCGGGCGGTCTATACGGTG
AAACTCGGTGGCGCCGTCTATGTGCTGCACTGTTTCCAGAAGAAATCGTGCCGCGGTATCGCGACGCCGAAGCCGGACAT
CGAACTGGTTCGCGCGCGCCTGAGGGCAGCCGAAGCACACGCAAATGGAGGAAAGTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11858.78 Da
• Isoelectric Point: 8.0686

>AT253680 WP_054317404.1 NZ_CP102458:2564236-2564559 [Burkholderia multivorans]
MIEIERGSGNVYADLGMPDAEEMRVKAQLAAKIAEIIKTRKWTQQHAARLLGMTQPKLSNMLRGQFRGVSEAKMLDCLAK
LGRDVQIVVGPDRHSETEGHIEVVFAA

Nucleotide

• Length: 324 bp

>AT253680 NZ_CP102458:2564236-2564559 [Burkholderia multivorans]
ATGATCGAAATCGAACGGGGCAGCGGCAACGTCTACGCGGATCTGGGCATGCCCGACGCCGAAGAAATGCGCGTCAAGGC
TCAACTCGCCGCCAAGATCGCGGAGATCATCAAGACTCGCAAATGGACGCAGCAGCACGCAGCACGATTGCTCGGCATGA
CGCAACCGAAGCTATCGAACATGCTGCGCGGGCAGTTTCGCGGCGTAAGCGAAGCGAAGATGCTCGACTGTCTCGCCAAG
CTCGGGCGCGATGTACAGATCGTCGTAGGACCGGACCGTCATTCGGAAACCGAAGGACACATCGAGGTCGTGTTCGCTGC
CTGA

Structures

Toxin

• AlphaFold DB: B9B4U7