Detailed information of TA system TA025367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 2971253..2971767
• Replicon: chromosome
• Accession: NC_015656
• Organism: Frankia symbiont of Datisca glomerata

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8B583
• Locus tag: FSYMDG_RS12350
• Protein ID: WP_013873793.1
• Coordinates: 2971507..2971767 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: F8B280
• Locus tag: FSYMDG_RS12345
• Protein ID: WP_013873792.1
• Coordinates: 2971253..2971510 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FSYMDG_RS12315	2966832..2968160	-	1329	WP_013873787.1	histidinol-phosphate transaminase	-
FSYMDG_RS12320	2968157..2969452	-	1296	WP_202797725.1	histidinol dehydrogenase	-
FSYMDG_RS12325	2969610..2969897	+	288	WP_013873789.1	hypothetical protein	-
FSYMDG_RS12330	2969897..2970277	+	381	WP_013873790.1	twitching motility protein PilT	-
FSYMDG_RS12340	2970562..2970798	-	237	WP_083828259.1	hypothetical protein	-
FSYMDG_RS24550	2970808..2971036	+	229	Protein_2429	hypothetical protein	-
FSYMDG_RS12345	2971253..2971510	+	258	WP_013873792.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
FSYMDG_RS12350	2971507..2971767	+	261	WP_013873793.1	Txe/YoeB family addiction module toxin	Toxin
FSYMDG_RS12355	2971951..2972751	-	801	WP_043606230.1	SAM-dependent methyltransferase	-
FSYMDG_RS12360	2973114..2974505	-	1392	WP_202797726.1	TIGR02679 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-70)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10228.60 Da
• Isoelectric Point: 7.2754

>T25367 WP_013873793.1 NC_015656:2971507-2971767 [Candidatus Frankia datiscae]
MKLSWTDHAWEDYLYWQTQDRRTLKRISTLIADIKRDPDGSGIGKPEILRNNLAGLRSRRIDDEHRLVYAVEPGEIIIIS
CRYHYE

Nucleotide

• Length: 261 bp

>T25367 NC_015656:2971507-2971767 [Candidatus Frankia datiscae]
TTGAAGCTCAGCTGGACCGACCACGCCTGGGAGGACTATCTCTACTGGCAGACCCAGGACCGCAGGACGCTCAAACGCAT
CAGCACCCTGATCGCTGATATCAAAAGGGACCCCGACGGTTCAGGTATCGGCAAGCCTGAGATTCTGCGTAACAACCTCG
CCGGTCTGCGCTCCCGCCGCATCGACGACGAGCACCGCCTCGTCTACGCAGTCGAACCCGGCGAGATCATCATCATCTCC
TGCCGTTACCACTACGAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9476.62 Da
• Isoelectric Point: 5.6864

>AT25367 WP_013873792.1 NC_015656:2971253-2971510 [Candidatus Frankia datiscae]
MTAAISASEARKTLFPLIERVNADHAPVEIVSKHGNAVLVSKEDWDSIMETNYLLRSPANAKRLTESIDQWRTGNARERD
LSPDA

Nucleotide

• Length: 258 bp

>AT25367 NC_015656:2971253-2971510 [Candidatus Frankia datiscae]
ATGACGGCCGCGATCAGTGCGAGCGAGGCGCGCAAGACCCTGTTCCCGCTCATCGAGCGGGTCAACGCCGACCACGCCCC
CGTCGAGATCGTTTCCAAGCACGGCAACGCGGTTCTGGTCAGCAAGGAGGACTGGGACTCGATCATGGAGACCAACTACC
TGCTACGCTCCCCCGCCAACGCCAAGCGACTGACGGAGAGTATCGACCAGTGGCGTACTGGTAACGCCAGGGAGCGCGAC
CTGAGCCCGGACGCTTGA

Structures

Toxin

• AlphaFold DB: F8B583

Antitoxin

• AlphaFold DB: F8B280