Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5119037..5119662 Replicon chromosome
Accession NZ_CP102438
Organism Klebsiella pneumoniae strain hvKP248

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NUJ53_RS24960 Protein ID WP_002882817.1
Coordinates 5119037..5119420 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NUJ53_RS24965 Protein ID WP_004150355.1
Coordinates 5119420..5119662 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUJ53_RS24945 (5116403) 5116403..5117305 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NUJ53_RS24950 (5117302) 5117302..5117937 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NUJ53_RS24955 (5117934) 5117934..5118863 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NUJ53_RS24960 (5119037) 5119037..5119420 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NUJ53_RS24965 (5119420) 5119420..5119662 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NUJ53_RS24970 (5119867) 5119867..5120784 + 918 WP_029884108.1 alpha/beta hydrolase -
NUJ53_RS24975 (5120798) 5120798..5121739 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NUJ53_RS24980 (5121784) 5121784..5122221 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NUJ53_RS24985 (5122218) 5122218..5123078 - 861 WP_002882807.1 virulence factor BrkB family protein -
NUJ53_RS24990 (5123072) 5123072..5123671 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T253666 WP_002882817.1 NZ_CP102438:c5119420-5119037 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT253666 WP_004150355.1 NZ_CP102438:c5119662-5119420 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References