Detailed information of TA system TA253665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4886502..4888183
• Replicon: chromosome
• Accession: NZ_CP102438
• Organism: Klebsiella pneumoniae strain hvKP248

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: NUJ53_RS23900
• Protein ID: WP_004146663.1
• Coordinates: 4886502..4887419 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: NUJ53_RS23905
• Protein ID: WP_004177809.1
• Coordinates: 4887425..4888183 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NUJ53_RS23880 (4881808)	4881808..4883328	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
NUJ53_RS23885 (4883325)	4883325..4884323	+	999	WP_004872882.1	ribose ABC transporter permease	-
NUJ53_RS23890 (4884354)	4884354..4885295	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
NUJ53_RS23895 (4885370)	4885370..4886377	+	1008	WP_004221949.1	carbohydrate kinase family protein	-
NUJ53_RS23900 (4886502)	4886502..4887419	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
NUJ53_RS23905 (4887425)	4887425..4888183	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
NUJ53_RS23910 (4888269)	4888269..4888997	-	729	WP_004177810.1	response regulator transcription factor	-
NUJ53_RS23915 (4889270)	4889270..4891417	+	2148	WP_012737173.1	formate dehydrogenase H subunit alpha, selenocysteine-containing	-
NUJ53_RS23920 (4891879)	4891879..4892565	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T253665 WP_004146663.1 NZ_CP102438:c4887419-4886502 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT253665 WP_004177809.1 NZ_CP102438:c4888183-4887425 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05