Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5057178..5057803 Replicon chromosome
Accession NZ_CP102435
Organism Klebsiella pneumoniae strain K194

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NR799_RS24995 Protein ID WP_002882817.1
Coordinates 5057178..5057561 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NR799_RS25000 Protein ID WP_004150355.1
Coordinates 5057561..5057803 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NR799_RS24980 (NR799_24980) 5054544..5055446 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NR799_RS24985 (NR799_24985) 5055443..5056078 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NR799_RS24990 (NR799_24990) 5056075..5057004 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NR799_RS24995 (NR799_24995) 5057178..5057561 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NR799_RS25000 (NR799_25000) 5057561..5057803 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NR799_RS25005 (NR799_25005) 5058008..5058925 + 918 WP_002882812.1 alpha/beta hydrolase -
NR799_RS25010 (NR799_25010) 5058939..5059880 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NR799_RS25015 (NR799_25015) 5059925..5060362 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NR799_RS25020 (NR799_25020) 5060359..5061219 - 861 WP_002882807.1 virulence factor BrkB family protein -
NR799_RS25025 (NR799_25025) 5061213..5061812 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T253644 WP_002882817.1 NZ_CP102435:c5057561-5057178 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT253644 WP_004150355.1 NZ_CP102435:c5057803-5057561 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References