253614

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5272598..5273223 Replicon chromosome
Accession NZ_CP102390
Organism Klebsiella pneumoniae strain K64

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NRF21_RS26065 Protein ID WP_002882817.1
Coordinates 5272598..5272981 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NRF21_RS26070 Protein ID WP_004150355.1
Coordinates 5272981..5273223 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRF21_RS26050 (NRF21_26050) 5269964..5270866 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NRF21_RS26055 (NRF21_26055) 5270863..5271498 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NRF21_RS26060 (NRF21_26060) 5271495..5272424 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NRF21_RS26065 (NRF21_26065) 5272598..5272981 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NRF21_RS26070 (NRF21_26070) 5272981..5273223 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NRF21_RS26075 (NRF21_26075) 5273428..5274345 + 918 WP_002882812.1 alpha/beta hydrolase -
NRF21_RS26080 (NRF21_26080) 5274359..5275300 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NRF21_RS26085 (NRF21_26085) 5275345..5275782 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NRF21_RS26090 (NRF21_26090) 5275779..5276639 - 861 WP_002882807.1 virulence factor BrkB family protein -
NRF21_RS26095 (NRF21_26095) 5276633..5277232 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T253614 WP_002882817.1 NZ_CP102390:c5272981-5272598 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT253614 WP_004150355.1 NZ_CP102390:c5273223-5272981 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References