Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4689899..4690709 Replicon chromosome
Accession NZ_CP102390
Organism Klebsiella pneumoniae strain K64

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag NRF21_RS23270 Protein ID WP_002887280.1
Coordinates 4689899..4690432 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag NRF21_RS23275 Protein ID WP_002887278.1
Coordinates 4690443..4690709 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NRF21_RS23265 (NRF21_23265) 4688730..4689851 + 1122 WP_002887282.1 cupin domain-containing protein -
NRF21_RS23270 (NRF21_23270) 4689899..4690432 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
NRF21_RS23275 (NRF21_23275) 4690443..4690709 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
NRF21_RS23280 (NRF21_23280) 4690812..4692245 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
NRF21_RS23285 (NRF21_23285) 4692235..4692918 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
NRF21_RS23290 (NRF21_23290) 4693090..4694475 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
NRF21_RS23295 (NRF21_23295) 4694493..4694837 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T253611 WP_002887280.1 NZ_CP102390:c4690432-4689899 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT253611 WP_002887278.1 NZ_CP102390:c4690709-4690443 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References