Detailed information of TA system TA025359

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 5517953..5518649
• Replicon: chromosome
• Accession: NC_015635
• Organism: Microlunatus phosphovorus NM-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MLP_RS24995
• Protein ID: WP_172641634.1
• Coordinates: 5517953..5518354 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MLP_RS25000
• Protein ID: WP_041790612.1
• Coordinates: 5518419..5518649 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLP_RS24985	5515945..5516748	+	804	WP_013866011.1	hypothetical protein	-
MLP_RS24990	5516745..5517824	+	1080	WP_013866012.1	hypothetical protein	-
MLP_RS24995	5517953..5518354	-	402	WP_172641634.1	VapC toxin family PIN domain ribonuclease	Toxin
MLP_RS25000	5518419..5518649	-	231	WP_041790612.1	hypothetical protein	Antitoxin
MLP_RS25005	5518771..5519010	+	240	WP_013866015.1	DUF433 domain-containing protein	-
MLP_RS27700	5519230..5519592	+	363	WP_156821295.1	hypothetical protein	-
MLP_RS25010	5519552..5520160	-	609	WP_013866017.1	alpha/beta hydrolase fold domain-containing protein	-
MLP_RS26885	5521038..5522933	-	1896	WP_013866018.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14583.72 Da
• Isoelectric Point: 6.8431

>T25359 WP_172641634.1 NC_015635:c5518354-5517953 [Microlunatus phosphovorus NM-1]
VNLLVALLHPAHVHHLQAQAWFTSVERFATTPITESGFIRMALNPAVAGQVTSLDDAMSSLNSVRADKRVEYVADDTSLA
LPTIDLVGLVGFRQVTDFHLVNLAACHSARLVTFDRKLAPTLAPEDRRFVHSL

Nucleotide

• Length: 402 bp

>T25359 NC_015635:c5518354-5517953 [Microlunatus phosphovorus NM-1]
GTGAACTTGCTTGTCGCGCTGTTGCATCCGGCTCACGTCCATCACCTCCAAGCGCAGGCATGGTTCACCTCGGTTGAGCG
GTTTGCCACCACGCCGATCACCGAGTCCGGGTTCATCCGGATGGCACTCAACCCCGCAGTCGCCGGCCAGGTCACCTCCC
TTGATGACGCCATGTCCTCGCTGAACTCCGTACGAGCCGACAAGCGAGTGGAGTACGTTGCCGATGACACCTCGCTCGCC
CTACCGACAATCGATCTCGTCGGACTCGTCGGATTCCGTCAGGTCACTGATTTCCACTTGGTCAATCTGGCTGCCTGCCA
CTCTGCTCGCCTGGTCACCTTCGATCGGAAGCTTGCCCCGACCCTCGCACCTGAGGACCGTCGGTTCGTCCACAGTCTTT
AG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 7745.72 Da
• Isoelectric Point: 4.3271

>AT25359 WP_041790612.1 NC_015635:c5518649-5518419 [Microlunatus phosphovorus NM-1]
MRTTLDLDPRVLTAARSRVNAGQNRSIGEAVSALALTGLATSSSSATTEANGLVLLPAAPGHVITDEMVAEALLDD

Nucleotide

• Length: 231 bp

>AT25359 NC_015635:c5518649-5518419 [Microlunatus phosphovorus NM-1]
ATGAGAACGACCCTCGACTTGGACCCGCGAGTCCTCACTGCGGCACGCTCCCGGGTGAACGCAGGCCAGAACAGGAGTAT
CGGCGAGGCTGTCTCTGCCTTGGCGCTGACTGGCCTGGCCACATCGAGCTCCTCGGCGACTACCGAGGCCAACGGACTGG
TTCTGCTTCCGGCAGCTCCGGGGCACGTCATCACCGACGAGATGGTTGCCGAGGCACTCCTTGACGACTGA