Detailed information of TA system TA025358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 4829591..4830072
• Replicon: chromosome
• Accession: NC_015635
• Organism: Microlunatus phosphovorus NM-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F5XTU7
• Locus tag: MLP_RS21900
• Protein ID: WP_013865380.1
• Coordinates: 4829809..4830072 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: F5XTU6
• Locus tag: MLP_RS21895
• Protein ID: WP_013865379.1
• Coordinates: 4829591..4829812 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLP_RS21860	4825936..4826682	+	747	WP_013865372.1	hypothetical protein	-
MLP_RS21865	4826679..4827512	+	834	WP_013865373.1	hypothetical protein	-
MLP_RS21870	4827466..4827762	+	297	WP_013865374.1	hypothetical protein	-
MLP_RS21875	4827753..4828106	-	354	WP_013865375.1	TfoX/Sxy family protein	-
MLP_RS21880	4828103..4828471	-	369	WP_013865376.1	nuclear transport factor 2 family protein	-
MLP_RS21885	4828533..4828967	-	435	WP_041793204.1	SRPBCC domain-containing protein	-
MLP_RS21890	4828960..4829364	-	405	WP_083843910.1	metalloregulator ArsR/SmtB family transcription factor	-
MLP_RS21895	4829591..4829812	+	222	WP_013865379.1	ribbon-helix-helix protein, CopG family	Antitoxin
MLP_RS21900	4829809..4830072	+	264	WP_013865380.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MLP_RS21905	4830107..4830913	+	807	WP_013865381.1	aminoglycoside phosphotransferase family protein	-
MLP_RS21910	4831226..4832077	-	852	WP_013865382.1	hypothetical protein	-
MLP_RS21915	4832202..4832627	-	426	WP_041790446.1	NAD(+)--rifampin ADP-ribosyltransferase	-
MLP_RS21920	4832672..4833001	+	330	WP_013865384.1	hypothetical protein	-
MLP_RS21925	4833068..4833595	-	528	WP_013865385.1	flavin reductase family protein	-
MLP_RS21930	4833592..4834152	-	561	WP_013865386.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (10-85)

Antitoxin

• Relaxosome protein TraY (4-40)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10132.60 Da
• Isoelectric Point: 9.9075

>T25358 WP_013865380.1 NC_015635:4829809-4830072 [Microlunatus phosphovorus NM-1]
VTHWHLETSPQFDKTARKLDQQTLHRVRAHLDQVCELEDPRTRGKGLTGKLAGYWRYRIGDYRVIVEIRDHALVIIAITI
GHRSGVY

Nucleotide

• Length: 264 bp

>T25358 NC_015635:4829809-4830072 [Microlunatus phosphovorus NM-1]
GTGACGCACTGGCATTTGGAGACCTCACCCCAGTTCGACAAAACGGCACGCAAGCTCGACCAACAGACACTGCATCGCGT
CCGGGCCCACCTTGACCAGGTCTGCGAACTCGAAGACCCACGCACTCGCGGCAAAGGACTGACGGGCAAGCTCGCCGGGT
ACTGGCGGTACCGGATCGGTGACTACCGCGTGATCGTGGAGATTCGCGACCACGCCTTGGTCATCATCGCGATCACGATC
GGGCACCGATCCGGTGTCTACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8559.49 Da
• Isoelectric Point: 4.2939

>AT25358 WP_013865379.1 NC_015635:4829591-4829812 [Microlunatus phosphovorus NM-1]
MPLSIRLTEDEEARLTALAERTGRSKTFYVRQAIEAYLEDLEEQYWADEAVREWEASGKRSRPAQQLWDELGV

Nucleotide

• Length: 222 bp

>AT25358 NC_015635:4829591-4829812 [Microlunatus phosphovorus NM-1]
ATGCCGTTGTCCATTCGGCTGACCGAAGACGAAGAAGCACGTCTGACCGCGCTCGCGGAGCGGACTGGTCGCTCAAAGAC
CTTCTATGTGCGCCAAGCCATCGAGGCCTATCTGGAGGACCTCGAAGAGCAGTACTGGGCTGATGAGGCCGTGCGCGAGT
GGGAGGCCTCGGGCAAGAGATCCCGTCCAGCTCAGCAGTTGTGGGATGAGCTGGGCGTGTGA

Structures

Toxin

• AlphaFold DB: F5XTU7

Antitoxin

• AlphaFold DB: F5XTU6