Detailed information of TA system TA025356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 4670309..4670940
• Replicon: chromosome
• Accession: NC_015635
• Organism: Microlunatus phosphovorus NM-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F5XSW4
• Locus tag: MLP_RS21070
• Protein ID: WP_013865206.1
• Coordinates: 4670309..4670728 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MLP_RS21075
• Protein ID: WP_041793154.1
• Coordinates: 4670719..4670940 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLP_RS21045	4665542..4666045	-	504	WP_049804619.1	GNAT family N-acetyltransferase	-
MLP_RS21050	4666130..4667803	-	1674	WP_172641610.1	hypothetical protein	-
MLP_RS21055	4668116..4668592	+	477	WP_013865203.1	DUF1877 family protein	-
MLP_RS21060	4668617..4669438	-	822	WP_013865204.1	sulfite exporter TauE/SafE family protein	-
MLP_RS21065	4669524..4670210	-	687	WP_156821248.1	GNAT family N-acetyltransferase	-
MLP_RS21070	4670309..4670728	-	420	WP_013865206.1	PIN domain nuclease	Toxin
MLP_RS21075	4670719..4670940	-	222	WP_041793154.1	ribbon-helix-helix protein, CopG family	Antitoxin
MLP_RS21080	4671135..4672208	-	1074	WP_013865208.1	redox-regulated ATPase YchF	-
MLP_RS21085	4672252..4673550	-	1299	WP_013865209.1	M18 family aminopeptidase	-
MLP_RS21090	4674239..4675603	+	1365	WP_156821469.1	DNA recombination protein RmuC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-127)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-34)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15240.56 Da
• Isoelectric Point: 4.7884

>T25356 WP_013865206.1 NC_015635:c4670728-4670309 [Microlunatus phosphovorus NM-1]
VAVTSWLLDKSAFVGLQLDQVVDAEVWKTRIDRGLVRISPITRLELGYSARTGVDGREGFRRPPVSLMPVERITPAIEDR
AEEVQMLLADRGQHRAPSIPDLLIAATAEKAGLTVLAVDKDFDLIAEVTGQPVETLALR

Nucleotide

• Length: 420 bp

>T25356 NC_015635:c4670728-4670309 [Microlunatus phosphovorus NM-1]
GTGGCGGTGACGAGTTGGCTGCTCGACAAGTCGGCGTTCGTGGGGCTCCAGCTCGACCAGGTCGTCGACGCCGAGGTGTG
GAAGACCAGGATTGATCGCGGTCTGGTGCGGATCTCGCCGATCACTCGTTTGGAGCTCGGGTACTCGGCACGGACGGGGG
TTGACGGGCGAGAGGGGTTCCGCCGGCCACCGGTCTCGCTGATGCCGGTGGAGCGGATCACGCCTGCGATTGAAGACAGG
GCTGAGGAGGTGCAGATGCTTCTTGCGGACCGAGGCCAGCATCGCGCGCCGTCAATCCCAGACCTGCTGATCGCCGCCAC
GGCTGAGAAGGCGGGCCTCACGGTGCTGGCCGTGGATAAGGACTTTGACCTGATCGCTGAGGTCACGGGGCAGCCGGTGG
AGACGCTCGCACTGCGCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8108.97 Da
• Isoelectric Point: 4.4230

>AT25356 WP_041793154.1 NC_015635:c4670940-4670719 [Microlunatus phosphovorus NM-1]
MTNVLIRGLSDSTVERIDAEASALGLSRNEFLRRKLEGDSPRSGETTTDENWRRAGEIFADLADPAVMADAWR

Nucleotide

• Length: 222 bp

>AT25356 NC_015635:c4670940-4670719 [Microlunatus phosphovorus NM-1]
ATGACGAACGTGCTCATACGAGGGCTCAGTGACTCAACCGTGGAACGGATCGATGCGGAGGCGTCAGCATTGGGGCTGTC
CCGCAACGAGTTCCTTCGCCGGAAGCTTGAGGGGGACAGTCCTCGGTCAGGCGAGACGACGACTGATGAGAACTGGCGGC
GTGCCGGTGAGATCTTTGCCGATCTTGCGGATCCAGCCGTGATGGCTGACGCGTGGCGGTGA

Structures

Toxin

• AlphaFold DB: F5XSW4