Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2910184..2910838 Replicon chromosome
Accession NZ_CP102379
Organism Escherichia coli strain BM28 lysU

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag NR350_RS14160 Protein ID WP_000244777.1
Coordinates 2910184..2910591 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NR350_RS14165 Protein ID WP_000354046.1
Coordinates 2910572..2910838 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NR350_RS14140 (2906141) 2906141..2907874 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
NR350_RS14145 (2907880) 2907880..2908590 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NR350_RS14150 (2908615) 2908615..2909511 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NR350_RS14155 (2909623) 2909623..2910144 + 522 WP_001055874.1 flavodoxin FldB -
NR350_RS14160 (2910184) 2910184..2910591 - 408 WP_000244777.1 protein YgfX Toxin
NR350_RS14165 (2910572) 2910572..2910838 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NR350_RS14170 (2911081) 2911081..2912061 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NR350_RS14175 (2912257) 2912257..2912916 - 660 WP_000250274.1 hemolysin III family protein -
NR350_RS14180 (2913080) 2913080..2913391 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
NR350_RS14185 (2913436) 2913436..2914869 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
NR350_RS14190 (2914926) 2914926..2915669 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T253551 WP_000244777.1 NZ_CP102379:c2910591-2910184 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT253551 WP_000354046.1 NZ_CP102379:c2910838-2910572 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References