Detailed information of TA system TA025355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 3983379..3984049
• Replicon: chromosome
• Accession: NC_015635
• Organism: Microlunatus phosphovorus NM-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MLP_RS17765
• Protein ID: WP_197536432.1
• Coordinates: 3983379..3983798 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F5XP54
• Locus tag: MLP_RS17770
• Protein ID: WP_013864543.1
• Coordinates: 3983810..3984049 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLP_RS26605	3978817..3979632	+	816	WP_013864537.1	glycosyltransferase family 2 protein	-
MLP_RS17745	3979723..3980622	-	900	WP_013864538.1	alpha/beta hydrolase	-
MLP_RS17750	3980615..3982351	-	1737	WP_013864539.1	hypothetical protein	-
MLP_RS17755	3982324..3983130	-	807	WP_013864540.1	Fpg/Nei family DNA glycosylase	-
MLP_RS17760	3983171..3983368	+	198	WP_013864541.1	hypothetical protein	-
MLP_RS17765	3983379..3983798	-	420	WP_197536432.1	type II toxin-antitoxin system VapC family toxin	Toxin
MLP_RS17770	3983810..3984049	-	240	WP_013864543.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MLP_RS17775	3984106..3984555	-	450	WP_041792883.1	ribose-5-phosphate isomerase	-
MLP_RS17780	3984708..3985538	+	831	WP_172641594.1	extensin family protein	-
MLP_RS17785	3985543..3986142	-	600	WP_041792885.1	DSBA oxidoreductase	-
MLP_RS17790	3986312..3988879	+	2568	WP_013864547.1	aminopeptidase N	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-126)

Antitoxin

• SpoVT-AbrB domain (5-33)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14161.88 Da
• Isoelectric Point: 5.4646

>T25355 WP_197536432.1 NC_015635:c3983798-3983379 [Microlunatus phosphovorus NM-1]
VTDPPVSVDTSVAVPLLVASHQHHDAVAEWARGRALSVSGHALAETYSVLTRLPGNSRVAPGDAVVLIDESFGGVLVLSA
DAAQTVHRELSGSGIAGGATYDGLMALAAREHGATLATRDARARATYEALGVAVEVVVT

Nucleotide

• Length: 420 bp

>T25355 NC_015635:c3983798-3983379 [Microlunatus phosphovorus NM-1]
GTGACCGATCCACCAGTGTCGGTGGACACGAGTGTCGCGGTGCCACTGCTGGTAGCTTCCCACCAGCACCACGACGCCGT
CGCTGAGTGGGCGCGTGGTCGCGCACTGAGTGTCAGCGGCCACGCACTCGCCGAGACGTATTCCGTTCTGACCAGGCTTC
CTGGCAATTCACGCGTCGCCCCCGGCGATGCCGTCGTGCTTATCGACGAGAGCTTCGGCGGGGTGCTGGTGTTGTCCGCC
GATGCAGCACAGACGGTTCACCGAGAGCTGTCCGGCAGCGGGATTGCCGGAGGCGCCACCTATGACGGCTTGATGGCACT
GGCTGCTCGTGAGCATGGCGCGACCCTGGCGACCAGGGACGCGCGGGCGCGGGCGACGTACGAGGCGTTGGGTGTTGCTG
TCGAGGTGGTCGTCACCTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8472.64 Da
• Isoelectric Point: 5.7973

>AT25355 WP_013864543.1 NC_015635:c3984049-3983810 [Microlunatus phosphovorus NM-1]
MEAIIDSGGRIVLPKHLRDALGLTPGTKVDISPYGTGVQVTPGGRTARLEREPDGRLVARGDVEVTDDLMYALIDSGRR

Nucleotide

• Length: 240 bp

>AT25355 NC_015635:c3984049-3983810 [Microlunatus phosphovorus NM-1]
ATGGAGGCCATCATCGACTCCGGGGGACGCATCGTGCTTCCCAAGCATCTTCGTGACGCGCTCGGCCTGACGCCCGGCAC
CAAGGTTGACATCTCGCCGTACGGGACCGGTGTGCAGGTGACACCTGGCGGGCGGACGGCCCGATTGGAGCGGGAGCCAG
ACGGTCGGCTCGTGGCGCGCGGCGACGTGGAGGTCACCGACGATCTGATGTACGCGCTGATCGACTCAGGTCGGCGGTGA

Structures

Antitoxin

• AlphaFold DB: F5XP54