Detailed information of TA system TA025354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 2687299..2687807
• Replicon: chromosome
• Accession: NC_015635
• Organism: Microlunatus phosphovorus NM-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F5XGN8
• Locus tag: MLP_RS12150
• Protein ID: WP_013863389.1
• Coordinates: 2687299..2687559 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: F5XGN9
• Locus tag: MLP_RS12155
• Protein ID: WP_013863390.1
• Coordinates: 2687556..2687807 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLP_RS12120	2682367..2682669	+	303	WP_013863383.1	helix-turn-helix domain-containing protein	-
MLP_RS12125	2682749..2683681	-	933	WP_013863384.1	ketopantoate reductase	-
MLP_RS12130	2683765..2684313	+	549	WP_041792151.1	TetR/AcrR family transcriptional regulator	-
MLP_RS12140	2684906..2685598	+	693	WP_049804533.1	MFS transporter	-
MLP_RS12145	2686228..2686869	-	642	WP_013863388.1	NAD(P)-binding domain-containing protein	-
MLP_RS12150	2687299..2687559	-	261	WP_013863389.1	Txe/YoeB family addiction module toxin	Toxin
MLP_RS12155	2687556..2687807	-	252	WP_013863390.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
MLP_RS12160	2688033..2688611	+	579	WP_197536548.1	tyrosine-protein phosphatase	-
MLP_RS26320	2688713..2689186	+	474	WP_049804534.1	NUDIX domain-containing protein	-
MLP_RS12170	2689274..2689993	+	720	WP_013863393.1	VOC family protein	-
MLP_RS12175	2689998..2690510	-	513	WP_013863394.1	hypothetical protein	-
MLP_RS26325	2690656..2691216	-	561	WP_013863395.1	hypothetical protein	-
MLP_RS12185	2691306..2692214	-	909	WP_013863396.1	TetR/AcrR family transcriptional regulator C-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10545.97 Da
• Isoelectric Point: 10.0226

>T25354 WP_013863389.1 NC_015635:c2687559-2687299 [Microlunatus phosphovorus NM-1]
VKVVFSTRGWDDYLWWQRQDRKLLNRINQLIRDVMRNGNEGIGKPEPLKHGFQGYWSRRINDEHRLVYKVVDDEVRVAAC
RYHYER

Nucleotide

• Length: 261 bp

>T25354 NC_015635:c2687559-2687299 [Microlunatus phosphovorus NM-1]
GTGAAGGTCGTCTTCAGCACTCGGGGCTGGGACGATTATCTGTGGTGGCAGCGGCAGGACCGCAAGCTCCTCAACCGGAT
CAACCAGCTGATCCGGGACGTCATGCGAAACGGCAATGAGGGCATCGGCAAGCCAGAGCCGCTGAAGCACGGTTTTCAGG
GCTATTGGTCTCGCCGGATCAACGACGAGCACCGTCTGGTCTACAAGGTTGTCGATGACGAGGTGCGGGTCGCGGCGTGC
CGCTACCACTACGAGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9330.52 Da
• Isoelectric Point: 4.4400

>AT25354 WP_013863390.1 NC_015635:c2687807-2687556 [Microlunatus phosphovorus NM-1]
MKTMSYTESRAKYAEVLDAVVNDREEVVITRAGHEPAVIVSLDDYESLKETAYLLRSPANARRLLASIDELESGKGVERE
LME

Nucleotide

• Length: 252 bp

>AT25354 NC_015635:c2687807-2687556 [Microlunatus phosphovorus NM-1]
ATGAAGACTATGAGCTACACGGAGTCTCGCGCAAAGTACGCCGAGGTGCTCGACGCGGTCGTCAACGACCGCGAGGAGGT
CGTCATCACACGGGCCGGGCACGAGCCTGCGGTCATCGTGTCACTGGACGACTATGAGTCCCTCAAGGAAACGGCGTACC
TGCTGCGCAGCCCAGCCAACGCGCGACGCCTGCTCGCATCCATCGACGAGCTCGAGTCTGGGAAAGGTGTGGAGCGGGAG
CTGATGGAGTGA

Structures

Toxin

• AlphaFold DB: F5XGN8

Antitoxin

• AlphaFold DB: F5XGN9