Detailed information of TA system TA025353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1039722..1040350
• Replicon: chromosome
• Accession: NC_015635
• Organism: Microlunatus phosphovorus NM-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: F5XMN8
• Locus tag: MLP_RS04590
• Protein ID: WP_013861846.1
• Coordinates: 1040012..1040350 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MLP_RS04585
• Protein ID: WP_013861845.1
• Coordinates: 1039722..1040021 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLP_RS04560	1035060..1036046	-	987	WP_197536569.1	NAD(P)-dependent alcohol dehydrogenase	-
MLP_RS04565	1036203..1036901	+	699	WP_013861841.1	TetR/AcrR family transcriptional regulator	-
MLP_RS04570	1036924..1037751	-	828	WP_172641539.1	alpha/beta fold hydrolase	-
MLP_RS04575	1037941..1038792	+	852	WP_013861843.1	CPBP family intramembrane metalloprotease	-
MLP_RS04580	1039148..1039501	-	354	WP_013861844.1	hypothetical protein	-
MLP_RS04585	1039722..1040021	-	300	WP_013861845.1	putative addiction module antidote protein	Antitoxin
MLP_RS04590	1040012..1040350	-	339	WP_013861846.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MLP_RS04595	1040451..1041347	-	897	WP_013861847.1	helix-turn-helix transcriptional regulator	-
MLP_RS04600	1041493..1042515	+	1023	WP_013861848.1	zinc-dependent alcohol dehydrogenase family protein	-
MLP_RS04605	1042575..1043816	+	1242	WP_156821348.1	multidrug effflux MFS transporter	-
MLP_RS04610	1043936..1044283	+	348	WP_013861850.1	hypothetical protein	-
MLP_RS04615	1044653..1045108	-	456	WP_197536502.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-94)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12777.57 Da
• Isoelectric Point: 9.5051

>T25353 WP_013861846.1 NC_015635:c1040350-1040012 [Microlunatus phosphovorus NM-1]
MLDVVRTDEFDSWLQRLKDQRGKSRILRRLDRLAQGNPGDVRPIGKGLSELRLNVGPGYRIYYLQDGEVLILLLCGGDKS
TQQKDIKKAHQLAAEWSADKQKAEEKEGTPWT

Nucleotide

• Length: 339 bp

>T25353 NC_015635:c1040350-1040012 [Microlunatus phosphovorus NM-1]
ATGCTCGACGTCGTCCGCACTGACGAATTCGACTCGTGGCTGCAGAGACTGAAAGACCAACGGGGCAAGTCCCGCATCCT
CCGCCGGCTCGACAGGCTAGCCCAGGGCAATCCGGGGGACGTCAGGCCGATCGGCAAAGGGTTATCGGAGCTCCGCCTCA
ATGTGGGTCCGGGATACCGGATCTACTACCTGCAAGACGGAGAAGTCTTGATCCTCCTCCTATGCGGCGGCGATAAGTCG
ACGCAGCAGAAGGACATCAAGAAGGCTCACCAACTGGCCGCGGAATGGTCGGCCGATAAGCAGAAGGCTGAAGAGAAGGA
GGGCACACCATGGACCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10857.28 Da
• Isoelectric Point: 4.6290

>AT25353 WP_013861845.1 NC_015635:c1040021-1039722 [Microlunatus phosphovorus NM-1]
MDLSEKYKKFDAADYLKDLDDVAAFLEGALEDSLEDPTAVPHALGIIARSQNMSELARRVGMSRDGLYKALSTDGNPTWS
TILKVTNALGLRFELHSVA

Nucleotide

• Length: 300 bp

>AT25353 NC_015635:c1040021-1039722 [Microlunatus phosphovorus NM-1]
ATGGACCTGAGCGAGAAGTACAAGAAATTCGATGCCGCCGACTACCTCAAGGACCTCGACGACGTCGCCGCGTTTCTGGA
AGGCGCCCTCGAAGACTCCCTCGAAGATCCGACCGCAGTTCCGCACGCACTGGGCATCATCGCCCGCTCGCAGAACATGA
GTGAACTCGCCCGTCGCGTCGGCATGAGCCGCGACGGCCTCTACAAGGCACTCTCCACGGACGGCAATCCCACCTGGTCG
ACCATCCTCAAGGTCACCAACGCCCTCGGACTCCGCTTCGAGTTGCACTCGGTCGCCTGA

Structures

Toxin

• AlphaFold DB: F5XMN8